Evidence map›Paper›PMID 42281996›Full record

ArticleResearch square2026

Single-nucleus multiomic atlas of ALS primary motor cortex nominates neuroprotective WDR49-expressing astrocytes.

Sam Bonsall, Rodrigo Siqueira Kazu, Marianne King, Diana Leung, Farah Y Mahiddine, J Robin Highley, Andrew Strange, Yongzhuo Chen, Matilde Sassani, Sara Cabras and 22 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors.

Sam BonsallSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Rodrigo Siqueira KazuSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Marianne KingSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Diana LeungArc Institute, Palo Alto, CA, USA.
Farah Y MahiddineSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.ORCID 0009-0001-7693-6062
J Robin HighleySheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Andrew StrangeSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Yongzhuo ChenDepartment of Biostatistics, University of Florida, Gainesville, FL, USA.
Matilde SassaniSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Sara CabrasSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Katie M BowdenSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Hollie E WareingSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Mahmoud K EldahshouryRita Levi Montalcini' Department of Neuroscience, University of Turin, Turin, Italy.
James R BoyneRita Levi Montalcini' Department of Neuroscience, University of Turin, Turin, Italy.
Mark CollinsSchool of Biosciences, University of Sheffield, Sheffield, UK.ORCID 0000-0002-7656-4975
Emma Marie MonteDepartment of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Calum HarveySheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Sarah B GornallSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Akanksha JangidSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Connie TreanorSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Tobias MollSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.ORCID 0000-0001-5928-2514
Charlotte H van DijkDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht, The Netherlands.
Yiliang HuangDepartment of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Alex E UrbanDepartment of Medicine, Stanford University School of Medicine, Stanford, CA, USA.ORCID 0000-0001-9772-933X
Pamela J ShawSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.ORCID 0000-0002-8925-2567
Ryan J H WestSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
Kevin P KennaDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht, The Netherlands.ORCID 0009-0001-2712-7218
Eran HornsteinDepartments of Molecular Genetics and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.ORCID 0000-0002-5534-1924
Sai ZhangDepartment of Biomedical Informatics & Data Science, Yale University School of Medicine, New Haven, CT, USA.ORCID 0000-0001-5996-6086
Johnathan Cooper-KnockSheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.ORCID 0000-0002-0873-8689
Jingtian ZhouArc Institute, Palo Alto, CA, USA.ORCID 0000-0003-2060-1922
Michael P SnyderDepartment of Medicine, Stanford University School of Medicine, Stanford, CA, USA.ORCID 0000-0003-0784-7987

Funding

Special EquipmentP50HG007735 · NHGRI · STANFORD UNIVERSITY · PI CHANG, HOWARD Y · 2014 to 2018
$15.2M
Leveraging Natural Phenotypic Variations of Heterogenous ALS Populations-in-a-Dish to Enable Scalable Drug DiscoveryR01NS131409 · NINDS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI GOODARZI, HANI, ICHIDA, JUSTIN KAWIKA · 2022 to 2025
$4.9M
NHGRI NIH HHS P50 HG007735NINDS NIH HHS R01 NS131409
6 · The paper itself

Abstract

Amyotrophic lateral sclerosis (ALS) causes selective neurodegeneration in primary motor cortex, yet cell-type-specific molecular changes driving this vulnerability remain poorly understood. We present an integrated single-nucleus RNA- and ATAC-sequencing atlas of 778,330 nuclei from the primary motor cortex of 140 genetically characterised donors. ALS is associated with widespread transcriptional reprogramming driven by a common set of transcription factors (TFs) across multiple cell-types. Astrocytes harbour the most differentially expressed genes. Within astrocytes, a WDR49-expressing subpopulation is spatially associated with TDP-43 pathology, and genetic variants within

Identifiers

PMID42281996
PMCPMC13252510

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.