Evidence map›Paper›PMID 42278326›Full record

ArticleInternational journal of molecular sciences2026

Genotoxicity Integration into Bioprocess Optimization Reveals Progressive DNA Damage During Bioreactor Expansion of Adipose-Derived Stem Cells.

Vinícius Augusto Simão, Rafaela Choi Peng So, Jaci Leme, Rafael Guilen de Oliveira, Gabriel Adan Araújo Leite, Luiz Gustavo de Almeida Chuffa, Aldo Tonso, João Tadeu Ribeiro-Paes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Vinícius Augusto SimãoDepartment of Biotechnology, School of Sciences, Humanities, and Languages, São Paulo State University (UNESP), Assis 19806-900, São Paulo, Brazil.ORCID 0000-0002-3915-2795
Rafaela Choi Peng SoDepartment of Biotechnology, School of Sciences, Humanities, and Languages, São Paulo State University (UNESP), Assis 19806-900, São Paulo, Brazil.
Jaci LemeLaboratory of Viral Biotechnology, Center for Development and Innovation, Butantan Institute, São Paulo 05503-900, São Paulo, Brazil.
Rafael Guilen de OliveiraDepartment of Biotechnology, School of Sciences, Humanities, and Languages, São Paulo State University (UNESP), Assis 19806-900, São Paulo, Brazil.
Gabriel Adan Araújo LeiteDepartment of Cell Biology, Embryology and Genetics, Center for Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis 88040-900, Santa Catarina, Brazil.ORCID 0000-0002-9437-6310
Luiz Gustavo de Almeida ChuffaDepartment of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, São Paulo, Brazil.ORCID 0000-0002-0199-3396
Aldo TonsoDepartment of Chemical Engineering, Polytechnic School, University of São Paulo (USP), São Paulo 05508-010, São Paulo, Brazil.ORCID 0000-0001-8498-7348
João Tadeu Ribeiro-PaesDepartment of Biotechnology, School of Sciences, Humanities, and Languages, São Paulo State University (UNESP), Assis 19806-900, São Paulo, Brazil.

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior CAPES - Finance Code 001Fundação de Amparo à Pesquisa do Estado de São Paulo Process numbers #2014/03324-4
6 · The paper itself

Abstract

Mesenchymal stromal cells derived from adipose tissue (ASCs) are widely used in regenerative medicine, requiring scalable expansion strategies that preserve both cellular function and biological quality. However, current bioprocess optimization approaches are primarily guided by proliferation and phenotypic stability, often overlooking genomic integrity as a critical attribute. In this study, we developed a stirred-tank bioreactor system for ASC expansion on microcarriers and applied a genotoxicity-informed optimization strategy by integrating growth kinetics, metabolic profiling, and DNA damage assessment across multiple operational conditions (B

Indexed as

Adipose TissueBioreactorsCell Culture TechniquesDNA DamageMesenchymal Stem CellsCell DifferentiationCell ProliferationCells, CulturedCell SurvivalHumansadipose-derived stem cellsbioreactorcomet assayDNA damagegenomic integritygenotoxicityhuman mesenchymal stromal cellsmetabolic profilingmicrocarrier culturemicronucleus test

Identifiers

PMID42278326
PMCPMC13256854

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.