Single-cell RNA sequencing reveals chemotherapy-induced apoptosis in acute myeloid leukemia via B cell depletion and M2 to M1 macrophage repolarization.

Di Zhou, Xue Bai, Chenchen Wang, Lintao Bi

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In one paragraph

Article in Human genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Di ZhouDepartment of Hematology and Oncology, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130000, Jilin, China.

Xue BaiDepartment of Hematology and Oncology, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130000, Jilin, China.

Chenchen WangDepartment of Hematology and Oncology, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130000, Jilin, China.

Lintao BiDepartment of Hematology and Oncology, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130000, Jilin, China. bilt@jlu.edu.cn.

Funding

BEIJING HONGYU MEDICAL DEVELOPMENT FOUNDATION Z610689157351172053BEIJING MEDICAL AWARD FOUNDATION ZkjcD105181352372093212

6 · The paper itself

Abstract

purposeThis study aimed to investigate the dynamic changes of immune microenvironment in acute myeloid leukemia (AML) during chemotherapy, thereby, exploring the mechanism of chemotherapy resistance of AML.

methodsSingle cell RNA sequencing (scRNAseq) was performed on 6 bone marrow samples from AML before or after chemotherapy (BC, AC) to obtain the fastq files. Bioinformatics analysis including GSEA, GSVA, pseudotime trajectory and cell chat analysis was used for constructing single-cell transcriptome and observing the dynamic changes of immune microenvironment. Western blot was carried out for validation.

resultsA total of 11 cell subpopulations were obtained, among which B cells, HSCs, and TAMs were the significant changed between BC and AC groups. MCM7 gene was highly expressed in malignant cells, co-expressed with CD34 + pre B cells in BC groups, and decreased in AC group. The SPINK2 + B cells activated JAK-STAT pathway. Furthermore, we found that chemotherapy driven the transition for M2 to M1 in TAMs polarization.

conclusionsMCM7 and SPINK2 were the targeted genes in AML during chemotherapy, which help for providing a theoretical basis for combined immunotherapy/targeted-therapy strategies.

Indexed as

ApoptosisB-LymphocytesLeukemia, Myeloid, AcuteMacrophagesHumansMinichromosome Maintenance Complex Component 7Sequence Analysis, RNASingle-Cell AnalysisSingle-Cell Gene Expression AnalysisTumor MicroenvironmentMCM7 protein, humanMinichromosome Maintenance Complex Component 7Acute myeloid leukemiaImmune microenvironmentMCM7Single cell RNA sequencingSPINK2

Identifiers

PMID42277943

DOI10.1186/s40246-026-01001-1

PMCPMC13483559

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.