Evidence map›Paper›PMID 42277808›Full record

ArticleBMC veterinary research2026

Establishment of triplex real-time quantitative PCR assay for African swine fever virus genotype I/II recombinants.

Zhuyun Sun, Jiezhen Guo, Yao Li, Tong Sun, Jingyi Liu, Yingnan Liu, Yuchen Nan, Hongjun Chen, Chunyan Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhuyun SunDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, China.
Jiezhen GuoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
Yao LiChina Agricultural University, Beijing100091, China.
Tong SunShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
Jingyi LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
Yingnan LiuChina Agricultural University, Beijing100091, China.
Yuchen NanDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, China.
Hongjun ChenChina Agricultural University, Beijing100091, China. vetchj@cau.edu.cn.
Chunyan WuDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, China. chunyanwu@nwsuaf.edu.cn.

Funding

China National Natural Science Foundation (General Project) 32570161
6 · The paper itself

Abstract

African swine fever (ASFV) leads a highly contagious and lethal hemorrhagic disease, causing a huge economic loss to the global pig industry. Recently, lethal genotype I/II recombinants were isolated and characterized in China and Vietnam. To differentiate the three genotypes (I, II and I/II recombinants) of ASFV in China, a triplex quantitative PCR (qPCR) assay was developed by targeting B646L, A151R and MGF360-14L genes. The detection limitation for the A151R, MGF360-14L, and B646L genes were 10 copies/μl, demonstrating a high sensitivity. Meanwhile, no cross-reactivity was observed with nucleic acids from ASFV genotype I and II or other swine viruses, confirming a high specificity for this assay. The coefficient of assay variation was below 2%, indicating an excellent repeatability. Importantly, when piglets were challenged with ASFV genotype I/II recombinant virus, the method could be used to detect the virus shedding as early as 3 dpi. In summary, a highly sensitive and specific detection method was established, which holds significant potential for rapid diagnosis and early warning of ASFV genotype I/II recombinant infection.

Indexed as

African Swine FeverAfrican Swine Fever VirusMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionAnimalsGenotypeRecombination, GeneticSensitivity and SpecificitySwineAfrican swine fever virusGenotype IGenotype IIGenotype I/II recombinantreal-time quantitative PCR

Identifiers

PMID42277808
PMCPMC13644039

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.