Evidence map›Paper›PMID 42277123›Full record

ArticleScientific reports2026

Reviving formalin-fixed, paraffin embedded (FFPE) tissues for on-slide and multiscale correlative microscopy.

Zenan Chen, Chad Moore, Samson Dowland, John DeWitt, Douglas J Taatjes, Gregory Milburn, Kenneth S Campbell, Amy Li, Filip Braet, Gerald J Shami

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zenan ChenSchool of Medical Sciences (Molecular Biomedicine Research Theme), The University of Sydney, Camperdown, NSW, 2006, Australia.
Chad MooreSydney Microscopy and Microanalysis, The University of Sydney, Camperdown, NSW, 2006, Australia.
Samson DowlandSchool of Medical Sciences (Molecular Biomedicine Research Theme), The University of Sydney, Camperdown, NSW, 2006, Australia.
John DeWittDepartment of Pathology & Laboratory Medicine, University of Vermont, Burlington, 05405, VT, USA.
Douglas J TaatjesMicroscopy Imaging Center, Center for Biomedical Shared Resources, Larner College of Medicine, University of Vermont, Burlington, 05405, VT, USA.
Gregory MilburnDivision of Cardiovascular Medicine, University of Kentucky, Lexington, KY, 40536, USA.
Kenneth S CampbellDivision of Cardiovascular Medicine, University of Kentucky, Lexington, KY, 40536, USA.
Amy LiSchool of Medical Sciences (Molecular Biomedicine Research Theme), The University of Sydney, Camperdown, NSW, 2006, Australia.
Filip BraetSchool of Medical Sciences (Molecular Biomedicine Research Theme), The University of Sydney, Camperdown, NSW, 2006, Australia.
Gerald J ShamiSchool of Medical Sciences (Molecular Biomedicine Research Theme), The University of Sydney, Camperdown, NSW, 2006, Australia. gerald.shami@sydney.edu.au.

Funding

Data-driven optimization of therapy for heart failureR01HL163977 · NHLBI · UNIVERSITY OF KENTUCKY · PI CAMPBELL, KENNETH S, LEE, LIK CHUAN · 2022 to 2025
$2.4M
American Heart Association 23TPA1074093Australian Research Council LE110100203NHLBI NIH HHS R01 HL163977NIH HHS HL163977
6 · The paper itself

Abstract

Correlative microscopy integrates complementary imaging modalities to enable structural analysis of biological samples across multiple length scales. Here, we present two correlative workflows for formalin-fixed, paraffin-embedded (FFPE) human tissues that extend the utility of archived clinical specimens for high-resolution imaging. The first, an on-slide correlative light and electron microscopy (CLEM) workflow, enables direct integration of brightfield light microscopy with scanning electron microscopy to resolve ultrastructural features from standard histological sections. The second, a multiscale three-dimensional workflow, combines X-ray micro-computed tomography with serial block-face scanning electron microscopy to enable targeted, volumetric ultrastructural analysis within a defined tissue context. Using these approaches, we demonstrate that FFPE tissues retain sufficient structural integrity to resolve cellular and subcellular architecture across liver, lung, brain, and heart specimens. Finally, we apply artificial intelligence-assisted image processing and segmentation to enable scalable, quantitative analysis of large-volume datasets. Together, these workflows establish FFPE specimens as a versatile and accessible resource for multiscale correlative imaging in both research and clinical settings.

Indexed as

MicroscopyParaffin EmbeddingTissue FixationFormaldehydeHumansImage Processing, Computer-AssistedLiverLungMicroscopy, Electron, ScanningX-Ray MicrotomographyFormaldehydeArtificial intelligenceCorrelative light and electron microscopyCorrelative microscopyFormalin-fixed paraffin embeddedHistopathologyVolume electron microscopy

Identifiers

PMID42277123
PMCPMC13507358

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.