Acoustic Cavitation-Induced Unfolding and Solubilization of Velvet Antler Protein for Antioxidant Peptide Release: Substrate Modification Kinetics, Quantum Chemistry, and Keap1/Nrf2-Associated Cellular Responses.

Xuanying Xin, Haowen Wang, Sungkwon Park, Changguo Yan, Qiang Li, Rui Du, Xiangzi Li

Abstract read

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Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Xuanying XinEngineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; College of Agriculture, Yanbian University, Yanji 133002, China.

Haowen WangEngineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; College of Agriculture, Yanbian University, Yanji 133002, China.

Sungkwon ParkDepartment of Food Science and Biotechnology, Sejong University, Seoul 05006, South Korea.

Changguo YanEngineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; College of Agriculture, Yanbian University, Yanji 133002, China.

Qiang LiEngineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; College of Agriculture, Yanbian University, Yanji 133002, China.

Rui DuEngineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; College of Agriculture, Yanbian University, Yanji 133002, China. Electronic address: duruijlau@163.com.

Xiangzi LiEngineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; College of Agriculture, Yanbian University, Yanji 133002, China. Electronic address: lxz@ybu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The targeted extraction of bioactive peptides from structurally robust biological matrices, such as velvet antler protein (VAP), is severely hindered by high steric hindrance and profound conformational stability. This study established a multiscale analytical framework to investigate ultrasound-assisted VAP modification and its association with antioxidant peptide release and Keap1/Nrf2-related cellular responses. High-intensity ultrasound pretreatment (optimized at 450 W, 30 min, 30 mL/g) effectively deconstructed the dense interfacial architecture of VAP, sub-micronizing the particle size to ∼175 nm and promoting an ultrasound-associated conformational transition from α -helices to random coils. Thermodynamic deconstruction revealed that acoustic shear forces significantly attenuated the denaturation enthalpy (ΔH) and elevated surface hydrophobicity (H

Indexed as

AntioxidantsAntlersKelch-Like ECH-Associated Protein 1NF-E2-Related Factor 2PeptidesUltrasonic WavesAnimalsKineticsMiceRAW 264.7 CellsSolubilityThermodynamicsAntioxidantsKelch-Like ECH-Associated Protein 1NF-E2-Related Factor 2PeptidesAcoustic cavitationAntioxidant peptidesKeap1-Nrf2 pathwayProtein unfoldingVelvet antler protein

Identifiers

PMID42276016

DOI10.1016/j.ultsonch.2026.107920

PMCPMC13276608

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.