ArticleIntensive care medicine experimental2026
Mass cytometry reveals complex neutrophil heterogeneity in patients with severe sepsis.
Article in Intensive care medicine experimental, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Nano-Enabled Microfluidic Platforms for Functional Immunomonitoring in Pediatric Sepsis.International journal of nanomedicine · 2026Review
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
rationaleSepsis is a state of life-threatening organ dysfunction in the setting of infection. It is biologically heterogeneous, as evidenced by whole blood transcriptomic analyses that reveal distinct molecular subtypes based on gene expression. At the cellular level, sepsis is primarily mediated by neutrophils, a leukocyte population increasingly recognized as heterogeneous across several domains. We sought to further characterize neutrophil heterogeneity by identifying neutrophil subsets in critically ill patients with severe sepsis based on multidimensional mass cytometry analysis.
methodsWe generated time series mass cytometry (CyTOF) data from whole blood samples taken from 17 patients with sepsis admitted to an intensive care unit who were enrolled in a randomized controlled trial of high-dose vitamin C. We analyzed these data using unsupervised machine learning techniques to identify distinct neutrophil subtypes. We characterized the resulting subtypes and described their changes over time.
resultsWe analyzed approximately 1.5 million cytometry events gated as neutrophils. We identified five clusters that reveal complex heterogeneity across multiple neutrophil markers of maturation and activation including olfactomedin-4 (OLFM4), CD177, glucose transporter 1 (GLUT1), CD16, and lipocalin-2. The two dominant clusters differed primarily in the abundance of OLFM4, a neutrophil granule protein. Between Day 1 and Day 7, there was an increased proportion of neutrophils in the dominant OLFM4-expressing cluster, a difference primarily driven by increased abundance of OLFM4 in the placebo group, but not the vitamin C group.
conclusionsOur findings point to complex multidimensional heterogeneity among neutrophils in sepsis, thereby extending the current concept of neutrophil heterogeneity with new data from critically ill patients with sepsis derived from mass cytometry. Variation in the temporal differences in cluster proportions between treatment arms may suggest opportunities for precision sepsis treatment.
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