Evidence map›Paper›PMID 42274797›Full record

ArticleBioprocess and biosystems engineering2026

Multivariate analysis of the effects of high seeding density on therapeutic protein production and sialylation in an intensified CHO cell culture process.

Hoon-Min Lee, Hyun-Seung Kim, Yeon-Gu Kim

Abstract read
PubMed Publisher
In one paragraph

Article in Bioprocess and biosystems engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hoon-Min LeeBiotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Hyun-Seung KimBiotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Yeon-Gu KimBiotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. ygkim@kribb.re.kr.

Funding

National Research Foundation of Korea (NRF) grant Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program
6 · The paper itself

Abstract

Intensified processes in Chinese hamster ovary (CHO) cell culture are increasingly implemented to enhance therapeutic protein production in biopharmaceutical manufacturing; however, their impact on glycan-related product quality attributes, particularly terminal sialylation, remains insufficiently characterized. In this study, a design of experiments approach combined with an Ambr

Indexed as

BioreactorsCell Culture TechniquesAnimalsCHO CellsCricetinaeCricetulusHydrogen-Ion ConcentrationMultivariate AnalysisN-Acetylneuraminic AcidN-Acetylneuraminic AcidCHO cell cultureDesign of experiments (DoE)High seeding densityProcess intensificationSialylationTherapeutic protein production

Identifiers

PMID42274797

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.