Evidence map›Paper›PMID 42274557›Full record

ArticleCells2026

Quantitative Assessment of GFAP-Based Astrocyte Morphology in the Cuprizone Model: A Comparative Evaluation of Neurolucida

Lukas Wenzel, Leo Heinig, Dongshi Wang, Elise Vankriekelsvenne, Nicole Wigger, Annelie Zimmermann, Johann Rößler, Tim Clarner, Markus Kipp

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lukas WenzelInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Leo HeinigInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Dongshi WangInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Elise VankriekelsvenneInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Nicole WiggerInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Annelie ZimmermannInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Johann RößlerInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.
Tim ClarnerAnatomy and Cell Biology, University Hospital Bonn, University of Bonn, Nussallee 10, 53115 Bonn, Germany.
Markus KippInstitute of Anatomy, Rostock University Medical Center, Gertrudenstr. 9, 18057 Rostock, Germany.ORCID 0000-0001-5043-9052

Funding

Deutsche Forschungsgemeinschaft KI 1469/15-1 and CL 665/6-1
6 · The paper itself

Abstract

Reactive astrocytes are a hallmark of several neurological diseases in multiple sclerosis and experimental demyelination models. Their morphological alterations are commonly assessed by qualitative histopathology, yet quantitative tools are required to better capture astrocytic heterogeneity and to allow correlations with imaging-derived biomarkers. Here, we present a workflow for the quantitative analysis of Glial Fibrillary Acidic Protein (GFAP) network remodeling in astrocytes in the cuprizone model of demyelination. C57BL/6 mice were intoxicated with cuprizone for 3 or 5 weeks to induce progressive demyelination, microglial activation, and reactive astrogliosis. Brain sections were processed for anti-GFAP immunohistochemistry, and individual astrocytes from the stratum oriens of the hippocampus were digitally reconstructed. Diverse parameters of GFAP topology, including soma size, process length, branching order, convex hull area, and ramification index, were extracted using either the commercial Neurolucida

Indexed as

AstrocytesCuprizoneGlial Fibrillary Acidic ProteinAnimalsDemyelinating DiseasesDisease Models, AnimalMaleMiceMice, Inbred C57BLCuprizoneGlial Fibrillary Acidic Proteinglial fibrillary astrocytic protein, mouseastrocytescuprizone modeldigital reconstructionGFAPhippocampusmorphologymultiple sclerosisNeurolucida® 360reactive gliosisSNT

Identifiers

PMID42274557
PMCPMC13257050

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.