Pangenomics for Agricultural Breeding: Construction Strategies, Evidence Integration, and Translational Constraints.

Pangenomics has become an important framework for representing genetic diversity beyond a single linear reference genome. In agricultural species, it improves access to structural variants (SVs), copy number variations (CNVs), presence/absence variations (PAVs), and non-reference regulatory or coding sequences that may contribute to domestication, adaptation, and breeding traits. This review summarizes recent progress in long-read sequencing, telomere-to-telomere (T2T) assembly, and graph-based genome analysis, with emphasis on both livestock and crop systems. We first define the conceptual boundary between pangenome representations and reference-based variant catalogs. We then compare three major technical routes: variant integration, reference-guided iterative graph construction, and reference-free graph construction. Their performance is evaluated in terms of accuracy, scalability, coordinate consistency, reference bias, computational demand, annotation transfer, and suitability for downstream breeding questions. We further discuss how pangenome resources support hidden variant discovery, QTL and GWAS interpretation, environmental adaptation analysis, and multi-omics-based candidate prioritization. Importantly, we highlight unresolved limitations, including graph complexity, pipeline-dependent SV calls, incomplete functional annotation, weak cross-study comparability, and the difficulty of distinguishing causal variants from linked or neutral variation. This review therefore treats pangenome studies as connected but non-equivalent evidence: resource-building studies establish representational breadth, method papers define technical feasibility, and trait-focused studies provide varying levels of biological support. Apparent inconsistencies among studies are interpreted as signals of differences in sampling, genome complexity, validation depth, and graph construction strategy rather than as simple disagreements.