ArticleFrontiers in cell and developmental biology2026
Development of rapid multiplex human herpesvirus detection systems based on recombinase polymerase amplification and a lateral flow assay.
Article in Frontiers in cell and developmental biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Human herpesviruses (HHVs) are highly prevalent globally (e.g., Epstein-Barr virus with over 95% prevalence) and can persist latently for life, causing severe conditions like blindness, encephalitis, and even lethal infections in neonates. HHV reactivation is of particular clinical concern in transplantation medicine, where immunosuppression can trigger viral reactivation, leading to graft rejection, secondary infections, and increased mortality. Notably, latently infected cells show no pathological changes, their contamination in cell cultures not only undermines the accuracy of scientific data and reliability of research results but also risks public health through the transmission of biological products. This study constructed three detection systems by combining multiplex Polymerase Chain Reaction (PCR) with Recombinase Polymerase Amplification (RPA) and Lateral Flow Assay (LFA): the Octaplex PCR system, with a sensitivity of 1 × 10
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