Evidence map›Paper›PMID 42272466›Full record

ArticleFundamental research2026

Pre-implantation embryo metabolism identified by PEMA reveals endogenous lactate insufficiency contributes to pre-implantation development arrest.

Jingyu Li, Weibo Hou, Qi Zhao, Haitao Xi, Weiwei Liu, Songling Xiao, Entong Song, Ling Zhu, Yizhen Cai, Ming Zong and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fundamental research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Jingyu LiChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.
Weibo HouOujiang Laboratory, Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
Qi ZhaoOujiang Laboratory, Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
Haitao XiDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Weiwei LiuChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.
Songling XiaoOujiang Laboratory, Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
Entong SongOujiang Laboratory, Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
Ling ZhuChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.
Yizhen CaiThe Second School of Medicine, Wenzhou Medical University, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Ming ZongChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.
Jiaming ZhangChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.
Xinyu WangClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Baicui WangOujiang Laboratory, Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
Liang ChengNHC Key Laboratory of Molecular Probes and Targeted Diagnosis and Therapy, College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, China.
Junzhao ZhaoDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Guoning HuangChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.
Qingran KongChongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University, Chongqing 400010, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Metabolism is a major regulator of pre-implantation embryonic development, but pre-implantation development arrest (PIDA) due to abnormal metabolisms remains ambiguous. By estimating a weight for each metabolic reaction using the translatome data, we developed a computational tool to perform pre-implantation embryos metabolic analysis (PEMA), and uncovered that the embryos exist in high activities of lactate dehydrogenase and lactate synthesis when major zygotic genome activation (ZGA) occurs in humans and mice. The insufficiency of endogenous lactate was predicted by PEMA in human 8-cell PIDA and corrected human tripronuclear (ch3PN) embryos, which was coordinated with failed H3K18lac and major ZGA. In transcriptomes, the human PIDA embryos resembled endogenous lactate-deprived mouse embryos. Lac-CoA addition could promote H3K18lac, major ZGA, and ch3PN pre-implantation development. Collectively, our study decodes the metabolic abnormalities in human PIDA embryos. The experimental validation suggests that PEMA offers an opportunity to study metabolic heterogeneity in early embryos.

Indexed as

Cell metabolismEndogenous lactateH3k18lacPre-implantation development arrestZygotic genome activation

Identifiers

PMID42272466
PMCPMC13247451

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.