Evidence map›Paper›PMID 42271259›Full record

ArticleBMC plant biology2026

Assembly and comparative analysis of the complete mitochondrial genome of hexaploid oat (Avena sativa).

Shiqiang Zhao, Ruohong Li, Zhicheng Jia, Tianqi Zhu, Chunjiao Mi, Juan Wang, Zhijia Lv, Peisheng Mao

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Shiqiang ZhaoCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Ruohong LiCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Zhicheng JiaCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Tianqi ZhuCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Chunjiao MiCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Juan WangCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Zhijia LvCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China.
Peisheng MaoCollege of Grassland Science and Technology, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing Municipality, Beijing, 100193, China. maops@cau.edu.cn.

Funding

China Agricultural Research System CARS-34
6 · The paper itself

Abstract

backgroundCommon oat (Avena sativa) is a globally important hexaploid cereal crop with a large and complex genome. While its nuclear and chloroplast genomes have been extensively characterized, the mitochondrial genome of hexaploid oat has remained unresolved, limiting our understanding of organellar evolution and mitochondrial function in this polyploid species.

resultsWe report the first complete assembly and comprehensive analysis of the A. sativa mitochondrial genome, which comprises two circular chromosomes totaling 634,262 bp with a GC content of 44.11%. The mitogenome encodes 78 genes, including 37 protein-coding genes (PCGs), 27 tRNAs, 12 rRNAs, and 2 pseudogenes. Extensive repeat sequences were identified, providing a structural basis for recombination and genome plasticity. Codon usage analysis revealed a strong AT bias and distinct codon preferences, while Ka/Ks analysis indicated predominant purifying selection on core OXPHOS genes, with possible signals of positive selection in mttB and nad7. A total of 463 RNA editing sites were predicted, predominantly enhancing protein hydrophobicity and potentially optimizing mitochondrial function. Comparative analyses revealed high collinearity with the diploid oat A. longiglumis and frequent transfers of chloroplast-to-mitochondrial DNA. Phylogenetic reconstruction based on 32 conserved mitochondrial genes robustly resolved relationships within Poaceae and its relatives.

conclusionsThe first complete mitogenome of hexaploid oat provides new insights into mitochondrial genome structure, evolution, and organellar genome dynamics in polyploid species, and constitutes a valuable genomic resource for future studies of mitochondrial biology, comparative genomics, and marker development in oats.

Indexed as

AvenaGenome, MitochondrialGenome, PlantPolyploidyPhylogenyRNA EditingAvena sativaMitochondrial genome assemblyMitogenomePoaceaeRNA editing

Identifiers

PMID42271259
PMCPMC13474782

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