ArticleCommunications biology2026
Single-cell transcriptomics reveals differences between chorionic and basal plate cytotrophoblasts and 2D-cultured trophoblast stem cells.
Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Creatine kinase regulates energy metabolism and growth of trophoblasts.bioRxiv : the preprint server for biology · 2026Article
- Functional heterogeneity in trophoblast stem cells derived from recurrent pregnancy loss products of conception.Molecular human reproduction · 2026Article
- Meteorin-like controls metabolic adaptations and physiological myocardial remodeling in pregnancy and lactation.Frontiers in endocrinology · 2026Article
- Stem cell models of human embryo implantation and trophoblast invasion.Current opinion in genetics & development · 2025Review
- Emerging models of human and non-human primate placental development - Centre for Trophoblast Research 17th annual meeting 2024.Biology open · 2024Review
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18 authors.
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Abstract
Cytotrophoblast (CTB) of the early gestation human placenta are bipotent progenitor epithelial cells, which can differentiate into invasive extravillous trophoblast (EVT) and multinucleated syncytiotrophoblast (STB). Trophoblast stem cells (TSC), derived from early first trimester placentas, have also been shown to be bipotential; however, their cell-of-origin has not been identified. In this study, we set out to probe the transcriptional diversity of early and late first trimester villous CTB (vCTB) and compare these to TSC. To this end, we performed single-cell RNA sequencing (scRNA-seq) on placental villous tissue from early (6-8 weeks) and late (12-14 weeks) first trimester placentas; we also evaluated CTB within basal (maternal) and chorionic (fetal) regions of early first trimester placenta, both by scRNA-seq and GeoMx-based spatial transcriptomics. Finally, we performed scRNA-seq on three TSC lines, derived from 6-8 week gestation placentas, as well as on early first trimester CTB at several timepoints during TSC derivation. We observed differences within CTB clusters associated with gestational age, further influenced by location near the basal or chorionic plates. We identified trophoblast states representing "initial state" vCTB (in vivo CTB progenitors), as well as additional CTB subtypes, precursor STB, and precursor and mature EVT. CTB progenitors appeared enriched in early first trimester placentas at the basal plate; additionally, basal plate CTB showed transcriptional features consistent with EVT bias, whereas chorionic plate CTB showed features associated with STB precursors. Clustering and trajectory inference analysis indicated that TSC were most like EVT precursor cells. In fact, vCTB lost their in vivo "initial state" markers, including PAGE4, as they transitioned to TSC during in vitro culture. This was confirmed by flow cytometric analysis of 6 different TSC lines, which showed uniform expression of proximal column markers ITGA2 and ITGA5. Additionally, we found that ITGA5
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