Evidence map›Paper›PMID 42270919›Full record

ArticleScientific reports2026

SERPINA3 and NDRG1 are critical diagnostic immune genes associated with macrophages in preeclampsia.

Zhuna Wu, Shihong Chen, Weihong Chen, Yajing Xie, Zhimei Zhou, Li Huang, Yueli Wang, Binbin Chen, Congmei Yang, Yumin Ke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhuna WuDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Shihong ChenDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Weihong ChenDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Yajing XieDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Zhimei ZhouDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Li HuangDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Yueli WangDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Binbin ChenDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China.
Congmei YangDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China. 99537310@qq.com.
Yumin KeDepartment of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian province, China. 398031313@qq.com.

Funding

Fujian Provincial Natural Science Foundation of China 2026J001695the Fujian Provincial Health Technology Project 2024GGA044the innovation of science and technology, Fujian province 2023Y9234the innovation of science and technology, Fujian province 2024Y9412the Second Affiliated Hospital of Fujian Medical University Doctoral Miaopu Project BS202401
6 · The paper itself

Abstract

Preeclampsia (PE) is a pregnancy complication involving immune dysregulation. This study aims to identify diagnostic immune biomarkers for PE using machine learning. mRNA expression profiles from GSE75010 and immune-related genes from ImmPort were analyzed. Differentially expressed immune-related genes (DIRGs) were identified and subjected to GO/KEGG enrichment, PPI network, LASSO, and mSVM-RFE analyses. Candidate biomarkers were validated in additional GEO datasets (GSE54618, GSE74341, GSE147776) and by RT-PCR, Western blot, and immunohistochemistry in placental tissues. Immune cell infiltration was evaluated using CIBERSORT. Ten DIRGs were identified between PE and normotensive pregnancies. GO/KEGG analyses highlighted cytokine-cytokine receptor interactions. SERPINA3 and NDRG1 were identified as diagnostic biomarkers (AUC = 0.812 and 0.866 in training set; 0.798 and 0.781 in test set). Elevated expression of SERPINA3 and NDRG1 was confirmed in PE placental tissues. Immune analysis revealed that both genes were negatively correlated with M2 macrophages. SERPINA3 and NDRG1 are critical immune-related diagnostic biomarkers for PE and are associated with M2 macrophage reduction, providing novel insights into PE pathogenesis and diagnosis.

Indexed as

Cell Cycle ProteinsIntracellular Signaling Peptides and ProteinsMacrophagesPre-EclampsiaSerpinsBiomarkersFemaleGene Expression ProfilingHumansN-myc Downstream-Regulated Gene 1 ProteinPlacentaPregnancyBiomarkersCell Cycle ProteinsIntracellular Signaling Peptides and ProteinsN-myc Downstream-Regulated Gene 1 ProteinSERPINA3 protein, humanSerpinsImmune genesMachine learningNDRG1PreeclampsiaSERPINA3

Identifiers

PMID42270919
PMCPMC13408773

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.