ArticleCell reports methods2026
FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.
Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- FLIMExplorer: interactive GUI for object-based visualization and analysis of fluorescence lifetime images.JPhys photonics · 2026Article
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- Autofluorescence imaging reveals the impact of cryopreservation on T cell metabolism and activation response.Molecular therapy. Advances · 2026Article
- Autofluorescence imaging reveals the impact of cryopreservation on T cell metabolism and activation response.bioRxiv : the preprint server for biology · 2025Article
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4 authors.
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Abstract
Fluorescence lifetime imaging microscopy (FLIM) is sensitive to molecular environments and enables high-resolution mapping of cellular heterogeneity. Yet, the journey from raw photon decays to biological insight remains fragmented by multi-step data extraction and siloed analyses, creating burdens for experts and non-experts alike. This work presents FLIM Playground, the first interactive graphical platform that unifies single-cell FLIM workflows. Modularly designed to encompass data extraction (if desired) and data analysis, FLIM Playground can check field-of-view metadata, calibrate and extract fluorescence lifetime features per region of interest along with morphology and texture features across channels, merge multiple datasets, and provide real-time visual analytic modules. Lifetime extraction was validated against commercial software and published results, and both data extraction and analysis were demonstrated on a FLIM dataset of cancer cell lines. By adopting best practices and offering interactivity, FLIM Playground promotes reproducibility, allows for expansion to new imaging modalities, and accelerates hypothesis-driven discovery.
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Registered trials
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