Protocol for ex vivo mouse paired single-gonad screening platform for phenotypical characterization.

Martín Andrés Estermann, Karina Flores Rodriguez, James Hunnicutt, Humphrey Hung-Chang Yao

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Martín Andrés EstermannReproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. Electronic address: martin.estermann@nih.gov.

Karina Flores RodriguezReproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.

James HunnicuttFabrication and Repair Studio, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.

Humphrey Hung-Chang YaoReproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. Electronic address: humphrey.yao@nih.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present an ex vivo paired single-gonad culture protocol using fetal mouse gonads, optimized for short-term screening with inhibitors, metabolites, or signaling modulators. We describe the experimental design, gonad dissection, paired culture setup, and treatment application to minimize variability. We further outline procedures for downstream phenotypic assessment, including immunostaining, multi-omics analyses, and metabolic measurements of gonadal tissue and culture media. For complete details on the use and execution of this protocol, please refer to Estermann et al.

Indexed as

GonadsAnimalsFemaleMaleMicePhenotypeCell-based AssaysCell BiologyCell cultureCell isolationCell separation/fractionationDevelopmental biologyGeneticsMetabolismMicroscopyModel OrganismsMolecular BiologyStem Cells

Identifiers

PMID42268724

DOI10.1016/j.xpro.2026.104628

PMCPMC13273873

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.