ArticleSTAR protocols2026
Protocol for synthesis and mechanical characterization of polyacrylamide hydrogels of varying stiffness for cell culture applications.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Protocol for the Preparation of Polyacrylamide Hydrogels with Tunable Stiffness and Their Application in Cell Culture, Live-Cell Imaging, and Fixed-Cell Immunofluorescence.Current protocols · 2026Article
- Quantitative Analysis of Cytoplasmic Viscosity in Colorectal Cancer Cells by Differential Dynamic Microscopy of Genetically Encoded Nanoparticles.Chemical & biomedical imaging · 2026Article
- Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics.Molecular & cellular proteomics : MCP · 2026Article
- Biological Models for Evaluating Hydrogel-Based Formulations in Wound Healing.Gels (Basel, Switzerland) · 2025Review
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Authors and funding
8 authors.
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Abstract
The impact of mechanical cues on cell behavior is increasingly being recognized, rendering hydrogel platforms that mimic the extracellular matrix indispensable in in vitro cell biology research. Here, we present a protocol for the synthesis and rheological characterization of polyacrylamide (PAAm) hydrogels with varying stiffnesses, produced as large, circular, unattached gels customizable in shape and size. We outline techniques for their use in cell culture and downstream applications involving secretome or cell analysis and protein visualization by fluorescence microscopy. For complete details on the use and execution of this protocol, please refer to Cresens et al.
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