Versatile vector tools for efficient protein screening across multiple expression systems.

Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu

Abstract read

In one paragraph

Article in FEBS open bio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Zhimin ZhuShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Yaqing LiuCollege of Light Industry and Food Engineering, Guangxi University, Nanning, China.

Lei QinDepartment of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Feng YuShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Qisheng WangShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Yahui LiuNational Key Laboratory of Non-food Biomass Energy Technology, Institute of Biology, Guangxi Academy of Sciences, Nanning, China.ORCID https://orcid.org/0000-0002-5983-0151

Funding

Guangxi Natural Science Foundation 2025GXNSFDA04240002National Key Research and Development Program of China 2017YFA0504901National Natural Science Foundation of China 42377012

6 · The paper itself

Abstract

Heterologous protein expression is a cornerstone of biological research, but screening across different expression systems is often labor-intensive. We developed versatile vector tools for E. coli, insect, and mammalian cells, offering His, MBP, and GST tags. These tools feature a standardized interface that enables rapid vector switching via homologous recombination, requiring only a single pair of primers for PCR amplification. This significantly accelerates gene construction, thereby greatly increasing the efficiency of parallel protein screening. We demonstrate successful protein expression and purification in various systems using these tools, showcasing their ability to streamline protein screening and improve efficiency. Our results highlight the versatility and effectiveness of these vector tools in facilitating rapid protein screening across multiple expression systems.

Indexed as

eukaryotic expression systemshigh‐throughput screeningmulti‐host protein expressionvector toolkit

Identifiers

PMID42268239

DOI10.1002/2211-5463.70262

PMCPMC13398956

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