Observational studyCritical care (London, England)2026
Monitoring T-cell function in septic shock: one-year experience with a fully automated assay.
Observational study in Critical care (London, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn sepsis, personalized immunotherapy is being evaluated as a strategy to restore immune function in the most severely affected patients. Biomarkers are critical in this process, as clear clinical indicators of immune status are lacking. Functional testing is considered the gold standard for assessing immune function, but its clinical implementation faces analytical and standardization challenges. The objective of the present prospective, observational, single-center cohort study was to evaluate a fully automated protocol for assessing T cell functionality in septic shock patients.
methodsIn 66 patients with septic shock, we assessed T lymphocyte functionality using an interferon-γ release assay (IGRA) in response to mitogen. The assay was performed via a fully automated protocol during a one-year period. Patients were monitored three times during the first week after intensive care unit (ICU) admission. Phenotypic immunological parameters, including T cell subpopulation counts and monocyte HLA-DR expression (mHLA-DR), were also assessed. Patient outcomes were followed for 28 days, and a composite clinical deterioration score was defined by the occurrence of 28-day mortality and/or ICU-acquired infection.
resultsCompared with reference values, we observed a significant reduction in IFN-γ release capacity, which correlated with characteristic alterations in cellular immunological parameters. By the end of the first week, reduced IFN-γ release combined with low mHLA-DR identified a severe immunological phenotype associated with an increased risk of clinical deterioration.
conclusionGiven the observational nature of this study, further well-designed investigations in larger patient cohorts are required to validate these findings and assess their potential clinical relevance. If confirmed, this fully automated assay (performed on whole blood, requiring no technician intervention, and providing results within four hours) may offer a practical tool for monitoring immune functional alterations in routine clinical practice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.