ArticleScientific reports2026
In situ buffering and enzymatic regulation of acidogenesis in two-stage anaerobic digestion of composite vegetable waste.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Peels of vegetable waste (VW), rich in cellulose, lignin, and hemicellulose, can impede hydrolysis due to reduced enzymatic accessibility and activity. This research comparatively evaluated the effects of composite vegetable waste (CVW) comprising of 2:3 food waste (FW):VW on biological buffering potential and enzymatic activity during acidogenesis in a two-stage (Leached Bed Reactor-airlift reactor) system. Results demonstrated that maximum buffering and enzymatic activity occurred within the first 3-5 days. The system maintained an in situ maximum buffering capacity of 0.145 mol/L (FW) and 0.089 mol/L (CVW) without chemical additives. Peak α-amylase activities reached 72.2 U/mL (FW) and 64 U/mL (CVW), while protease reached 34.94 U/mL (CVW). The ratio of 2:3 for FW:VW moderated enzymatic hydrolysis rates through its lignocellulosic content and stabilized the acidogenic environment, which in turn regulated VFA dynamics, resulting in a 1.8-fold increase in acetate yield (18.19 g/L). Conversely, FW achieved a higher biohydrogen yield of 121.3 mL/g VS (43% composition) compared to 110 mL/g VS (36.4% composition) for CVW. The FW also produced 4.2 times higher propionic acid (95.3 g/L) than CVW (22.64 g/L). Life Cycle Assessment (LCA) confirmed the sustainability of this minimized-discharge model, with H
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