Evidence map›Paper›PMID 42262762›Full record

ArticleVirulence2026

The 3'Untranslated region is a critical determinant of Getah virus replication, pathogenesis, and vector competence.

Tongwei Ren, Peijie Li, Muyang Liu, Liping Zhang, Zhen Zhong, Guowei Wang, Xindong Wang, Lingshan Zhou, Yifeng Qin, Kang Ouyang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virulence, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Tongwei RenLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Peijie LiLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Muyang LiuLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Liping ZhangLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Zhen ZhongLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Guowei WangLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Xindong WangLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Lingshan ZhouLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Yifeng QinLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.ORCID 0000-0002-2486-6154
Kang OuyangLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.ORCID 0000-0003-2082-2362
Yeshi YinLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Ying ChenLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.ORCID 0000-0001-6720-353X
Weijian HuangLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Zuzhang WeiLaboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.ORCID 0000-0002-6317-2315

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Getah virus (GETV), a mosquito-borne arbovirus of the Alphavirus genus, poses an emerging threat to livestock economies and public health, underscored by its expanding host range and association with recent outbreaks of heightened virulence. While the functional significance of the 3' untranslated region (3'UTR) in alphavirus biology is recognized, its specific role in GETV remained undefined. Herein, we elucidate the virological functions of the GETV 3'UTR through a reverse genetics approach, generating a panel of viruses with targeted deletions. We demonstrate that the GETV 3'UTR is remarkably plastic, tolerating a consecutive deletion of up to 310 nucleotides while remaining viable. Deletion of conserved repeat sequence elements (RSEs) induced a cell-type-specific replication deficiency in vitro and significantly attenuated virulence in a murine model. A comprehensive deletion mutant (rGETV-KO310) exhibited further impaired replication kinetics in vitro and was profoundly attenuated in vivo, eliciting only transient morbidity with no mortality in both neonatal and weaned mice. Furthermore, this mutant displayed a significant defect in early colonization within mosquito vectors, indicating a role in vector competence. Comparative transcriptomic profiling of knee joints revealed that attenuation correlates with the altered modulation of critical host immune responses, notably the interferon and MAPK signaling pathways. Collectively, these findings establish the GETV 3'UTR as a pivotal regulator of viral fitness, pathogenesis, and transmission. This work provides a foundational rationale for the strategic development of live-attenuated vaccine candidates based on targeted 3'UTR attenuation.

Indexed as

3' Untranslated RegionsAlphavirusAlphavirus InfectionsMosquito VectorsVirus ReplicationAnimalsCell LineFemaleMiceSequence DeletionVirulence3' Untranslated Regions3’ untranslated regionGetah virusmutational analysispathogenicitytransmission potentialviral replication

Identifiers

PMID42262762
PMCPMC13285573

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.