Evidence map›Paper›PMID 42262439›Full record

ArticlePlant cell reports2026

Genome-wide characterization of MADS-box genes reveals CsaSOC1 as a key regulator of flowering in Cannabis sativa L.

Shuyao Li, Qian Zhao, Fu Wang, Ruidong Sun, Michael K Deyholos, Peng Di, Xiujuan Lei, Yingnan Liu, Jian Zhang

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Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Shuyao Li *Faculty of Agronomy, Jilin Agricultural University, Changchun, 130118, China.
Qian Zhao *Faculty of Agronomy, Jilin Agricultural University, Changchun, 130118, China.
Fu WangFaculty of Agronomy, Jilin Agricultural University, Changchun, 130118, China.
Ruidong SunFaculty of Agronomy, Jilin Agricultural University, Changchun, 130118, China.
Michael K DeyholosChangchun International Laboratory of Fiber Plant Biotechnology, Changchun, 130108, China.
Peng DiCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China.
Xiujuan LeiCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China. xiujuanl@jlau.edu.cn.
Yingnan LiuNational and Local Joint Laboratory of Wetland and Ecological Conservation, Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin, 150040, China. liuyingnan@hljas.cn.
Jian ZhangFaculty of Agronomy, Jilin Agricultural University, Changchun, 130118, China. jian.zhang@ubc.ca.

Funding

Jilin Agricultural University high-level researcher grant JLAUHLRG20102006Jilin Provincial Department of Human Resources and Social Security Grant No.201020012The 111 Project, Northeast Advantageous Characteristic Resources and Health Food Discipline Innovation Introduction Base No: D23007
6 · The paper itself

Abstract

key messageThis study identified 57 CsaMADS genes in Cannabis sativa L., characterized the expression and homology of key candidates CsaSOC1 and CsaFUL, and confirmed their protein interaction, thereby providing a molecular basis for investigating flowering regulation. MADS-box transcription factors are central regulators of plant growth and development, particularly in floral morphogenesis and the control of flowering time. In this study, we identified 57 MADS-box genes in hemp (Cannabis sativa L.) and classified them into Type I (22 genes) or Type II (35 genes) groups, which were further categorized into 15 distinct subfamilies. Genes within the same subfamily exhibited similar exon-intron structures and highly conserved protein motifs. Evolutionary analysis of MADS-box family members revealed that seven tandem and seven segmental duplication events had contributed to the expansion of the MADS-box gene family in hemp. Promoter analysis uncovered numerous cis-acting elements associated with light responsiveness, phytohormone signaling (including auxin and gibberellin), and environmental stress responses. Transcriptomic profiling showed that most CsaMADS-box genes are highly expressed in floral tissues, supporting their roles in reproductive development. Notably, a SOC1-like gene, CsaSOC1, was identified as a potential flowering integrator, and its physical interaction with CsaFUL was confirmed by yeast two-hybrid and luciferase complementation assays. Together, these results provide a comprehensive genomic overview of the MADS-box gene family in hemp and establish a valuable foundation for future functional studies of flowering regulation.

Indexed as

CannabisFlowersMADS Domain ProteinsPlant ProteinsGene Expression ProfilingGene Expression Regulation, PlantGenome, PlantMultigene FamilyPhylogenyPlant Growth RegulatorsPromoter Regions, GeneticMADS Domain ProteinsPlant Growth RegulatorsPlant ProteinsCannabis sativa L. (hemp)Expression profilingFlowering regulationGene duplicationGenome-wide analysisMADS-box transcription factorsProtein–protein interaction

Identifiers

PMID42262439

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