Evidence map›Paper›PMID 42262233›Full record

ArticlePharmacology research & perspectives2026

Using a NanoBRET-Based Ligand-Binding Assay at the β

Abigail Pearce, Xianglin Huang, Theo Redfern-Nichols, Claudia M Sisk, Dewi Safitri, Michael Collins, Milena A Malcharek, Sergio Tomey García, Chiharu Nakamura, Andrzej Harris and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmacology research & perspectives, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Abigail PearceDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0001-9845-0541
Xianglin HuangDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0009-0003-2947-7638
Theo Redfern-NicholsDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0009-0000-7503-4510
Claudia M SiskDepartment of Pharmacology, University of Cambridge, Cambridge, UK.
Dewi SafitriDepartment of Pharmacology, University of Cambridge, Cambridge, UK.
Michael CollinsDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0009-0004-3230-5658
Milena A MalcharekDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0009-0000-3968-2385
Sergio Tomey GarcíaDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0009-0008-6475-7631
Chiharu NakamuraDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0009-0005-0547-7267
Andrzej HarrisDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0003-4885-7401
Graham LaddsDepartment of Pharmacology, University of Cambridge, Cambridge, UK.ORCID https://orcid.org/0000-0001-7320-9612

Funding

Biotechnology and Biological Sciences Research Council BB/V509334/1Biotechnology and Biological Sciences Research Council BB/W014831/1Biotechnology and Biological Sciences Research Council BB/X010899/1Biotechnology and Biological Sciences Research Council BB/Y513817/1Biotechnology and Biological Sciences Research Council BB/Z516454/1Cambridge TrustChina Scholarship CouncilRoyal Society INF/R2/212001
6 · The paper itself

Abstract

The teaching of pharmacology includes a fundamental understanding of ligand binding; how it is measured and how it is calculated. We sought to modernize the techniques by which ligand affinity is determined by undergraduate students and introduce them to parameter estimation through curve fitting. We taught a NanoBRET ligand binding assay to student cohorts completing their second year of undergraduate study in Natural Sciences (71 and 61 students in 2024 and 2025 respectively). The aim was to measure the affinity of fluorescent and unlabeled ligands for the β

Indexed as

PharmacologyReceptors, Adrenergic, beta-2HumansLigandsProtein BindingStudentsLigandsReceptors, Adrenergic, beta-2G protein‐coupled receptorK dNanoBRET bindingteachingundergraduateβ2‐adrenoceptor

Identifiers

PMID42262233
PMCPMC13248772

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.