ArticlePlant, cell & environment2026
CHG Hypomethylation of BoCYP83A1 May Contribute to Methyl Jasmonate-Induced Sulforaphane Accumulation in Broccoli.
Article in Plant, cell & environment, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- CHG Hypomethylation of BoCYP83A1 May Contribute to Methyl Jasmonate-Induced Sulforaphane Accumulation in Broccoli.Plant, cell & environment · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Sulforaphane (SFN) is widely recognized for its health-promoting benefits. While several studies have reported that methyl jasmonate (MeJA) enhances SFN accumulation, the role of epigenetic regulation remains unclear. Here, we performed an integrated analysis of transcriptome, whole-genome bisulfite sequencing, and small RNA-seq data from broccoli florets and leaves under MeJA treatment and control conditions. Our results revealed that MeJA-responsive differentially methylated regions (DMRs) predominantly occurring in the CHG context. Integrative analysis of methylation and siRNA data indicated that changes in MeJA-responsive CHH methylation were associated with 24-nucleotide siRNA clusters. Furthermore, inhibiting DNA methylation using 5-azacytidine treatment in broccoli seedlings increased SFN content, validating the role of DNA hypomethylation in this process. By combining transcriptional regulatory network analysis with DMR data, we identified the MeJA-responsive CHG-hypomethylated gene BoCYP83A1. The McrBC-PCR assay confirmed reduced methylation in the BoCYP83A1 promoter region. The function of BoCYP83A1 in MeJA-induced SFN biosynthesis was validated using RNA interference in broccoli. Further evidence demonstrated that the transcription factors BoMYB68 and BoIAA18 promote BoCYP83A1 expression by directly binding to its promoter. Our findings not only provide novel insights into the epigenetic regulation underlying MeJA-induced SFN accumulation in plants but also facilitate molecular breeding for the development of high-SFN crops.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.