Evidence map›Paper›PMID 42260952›Full record

ArticleMedical and veterinary entomology2026

Flea infestation and molecular detection of Rickettsia and Bartonella spp. in domestic dog-associated fleas from the inter-Andean basin of Cundinamarca, Colombia.

Carlos Ramiro Silva-Ramos, Alejandro Ramírez-Hernández, Nicole L Mendell, Laura Natalia Robayo-Sánchez, Jerson Andrés Cuéllar-Sáenz, Jesús A Cortés-Vecino, J Manuel Matiz-González, Marylin Hidalgo, Lucas S Blanton, David H Walker

Abstract read
In one paragraph

Article in Medical and veterinary entomology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Carlos Ramiro Silva-RamosGrupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.ORCID https://orcid.org/0000-0003-0669-494X
Alejandro Ramírez-HernándezUniversidad de La Salle, Bogotá D.C., Colombia.ORCID https://orcid.org/0000-0001-8689-5930
Nicole L MendellDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Laura Natalia Robayo-SánchezGrupo de Investigación Parasitología Veterinaria, Laboratorio de Parasitología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá D.C., Colombia.
Jerson Andrés Cuéllar-SáenzGrupo de Investigación Parasitología Veterinaria, Laboratorio de Parasitología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá D.C., Colombia.
Jesús A Cortés-VecinoGrupo de Investigación Parasitología Veterinaria, Laboratorio de Parasitología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá D.C., Colombia.
J Manuel Matiz-GonzálezMolecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia.
Marylin HidalgoGrupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Lucas S BlantonDepartment of Internal Medicine-Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA.
David H WalkerDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.ORCID https://orcid.org/0000-0002-3309-4360

Funding

Research Training Program on the Impact of Zoonotic and Vector-borne Viruses, Rickettsiae, and Leptospira in Acute Undifferentiated Febrile IllnessesD43TW010331 · FIC · UNIVERSITY OF TEXAS MED BR GALVESTON · PI AGUILAR, PATRICIA VERONICA, RODAS, JUAN DAVID · 2016 to 2025
$2.6M
FIC NIH HHS D43 TW010331Fogarty International Center of the National Institutes of Health D43TW010331Universidad Nacional de Colombia 41525
6 · The paper itself

Abstract

Dogs are common companion animals, often infested by fleas that can transmit zoonotic pathogens in rural tropical areas. In Colombia, studies on flea-borne agents in dogs are limited. Understanding the presence of these ectoparasites and associated microorganisms is crucial. To assess flea infestation and detect and identify Rickettsia and Bartonella spp. in fleas collected from dogs in Cundinamarca, Colombia. Between 2019 and 2020, fleas were collected from rural domestic dogs in 24 municipalities of western Cundinamarca. Fleas were morphologically identified and pooled for DNA extraction. Molecular screening of Rickettsia and Bartonella spp. was performed by qPCR. Positive samples underwent further characterization by conventional PCR. Rickettsia was additionally analyzed using RFLP, while Bartonella was evaluated through phylogenetic analysis. Of the 116 dogs examined across 24 municipalities, 39.7% were infested with fleas. A total of 138 fleas were collected and grouped into 46 pools, with Ctenocephalides felis felis identified in 94% of them. Rickettsia was detected in 83% of pools, with Rickettsia asembonensis and Rickettsia felis identified in most positive samples. Bartonella was found in 28% of pools; however, sequencing success was limited, and no species-level identification could be achieved. This study documents flea infestation in domestic dogs in Cundinamarca, predominantly by C. felis felis. High detection of R. asembonensis and presence of R. felis confirm widespread rickettsial circulation in fleas. Bartonella was detected less frequently, but species-level identification was not achieved.

Indexed as

BartonellaceaecaninesColombiaRickettsiaceaeSiphonapteravector‐borne agents

Identifiers

PMID42260952
PMCPMC13531667

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.