Evidence map›Paper›PMID 42258749›Full record

ArticleJCI insight2026

Serum starvation drives ALIX-dependent extracellular vesicle biogenesis and determines tumor progression.

Xueqiang Peng, Jiaxing Liu, Guolong Zeng, Yafei Xiao, Zhixiong Hao, Guangpeng He, Hongyuan Jin, Yu Gao, Shilei Tang, Shibo Wei and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xueqiang PengDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Jiaxing LiuDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Guolong ZengDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Yafei XiaoDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Zhixiong HaoDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Guangpeng HeDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Hongyuan JinDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Yu GaoDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Shilei TangDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Shibo WeiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Yan LiDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Yifan YuDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Liang YangDepartment of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Hangyu LiShenyang Key Laboratory for Biomedical and Intelligent Mesh, Shenyang, Liaoning, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tumor cells are constantly confronted with nutrient deprivation; however, the effect of serum starvation on the remodeling of endosomal compartments and extracellular vesicles (EVs) in tumor cells remains unclear. Here, we found that serum starvation pronouncedly promotes multivesicular body (MVB) biogenesis, EV formation, and cargo selection. Specifically, by generating a constitutively active Rab5Q79L mutant to induce the enlargement of MVB, we revealed for the first time to our knowledge that ANXA3 is sorted into intraluminal vesicles (ILVs) of MVB. Mechanistically, we confirmed that serum starvation regulates the endosomal sorting complex required for transport-associated (ESCRT-associated) protein ALG-2 interacting protein X (ALIX), which recruits ESCRT-III to MVB and binds to annexin A3 (ANXA3) to mediate its sorting into ILVs of MVB. Our study highlights that serum starvation promotes an ALIX-dependent ESCRT-III recruitment pathway, which loads protumor ANXA3 cargo to exert a profound effect on tumor progression.

Indexed as

Calcium-Binding ProteinsCell Cycle ProteinsEndosomal Sorting Complexes Required for TransportExtracellular VesiclesNeoplasmsAnimalsCell Line, TumorDisease ProgressionHumansMultivesicular Bodiesrab5 GTP-Binding ProteinsCalcium-Binding ProteinsCell Cycle ProteinsEndosomal Sorting Complexes Required for TransportPDCD6IP protein, humanrab5 GTP-Binding ProteinsCancerCell biologyOncology

Identifiers

PMID42258749
PMCPMC13313550

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.