Evidence map›Paper›PMID 42257177›Full record

ArticlemicroPublication biology2026

Two-photon characterisation of long-Stokes-shift dye ATTO 490LS for single-laser multicolour imaging.

King Yee Cheung, Yue Wu, Shu Ying Lee, Xianyuan Zhang, Masahiro Fukuda, Danesha Devini Suresh, Adam Claridge-Chang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

King Yee CheungProgramme in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore.
Yue WuMicroscopy Core Facility - Multiphoton Microscopy Unit, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Shu Ying LeeMicroscopy Core Facility - Multiphoton Microscopy Unit, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Xianyuan ZhangProgramme in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore.
Masahiro FukudaProgramme in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore.
Danesha Devini SureshProgramme in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore.
Adam Claridge-ChangProgramme in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long-Stokes-shift fluorophores enable high sensitivity and multiplexed imaging with single-wavelength excitation. Under single-photon illumination ATTO 490LS exhibits a 165-nm Stokes shift, but its two-photon properties remain uncharacterised. Emission and excitation spectral analyses of ATTO 490LS in ex vivo

Identifiers

PMID42257177
PMCPMC13237719

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.