Evidence map›Paper›PMID 42255625›Full record

ArticleACS omega2026

Structure-Guided Affinity Maturation of an Antibody Fragment for Gold-Surface Biosensing of N‑Terminal Pro-B-Type Natriuretic Peptide.

Kanchana Monkhang, Jarumas Jitwasinkul, Nathjanan Jongkon, Permkun K Permsirivisarn, Thomanai Lamtha, Kiattawee Choowongkomon, Lueacha Tabtimmai, Napachanok M Swainson

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Article in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kanchana MonkhangDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Jarumas JitwasinkulDepartment of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology, North Bangkok, Bangkok 10800, Thailand.
Nathjanan JongkonDepartment of Social and Applied Science, College of Industrial Technology, King Mongkut's University of Technology, North Bangkok, Bangkok 10800, Thailand.
Permkun K PermsirivisarnDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Thomanai LamthaDepartment of Clinical Chemistry, Faculty of Medical Technology, Nation University, Lampang 52000, Thailand.ORCID https://orcid.org/0000-0001-5918-8704
Kiattawee ChoowongkomonDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.ORCID https://orcid.org/0000-0002-2421-7859
Lueacha TabtimmaiDepartment of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology, North Bangkok, Bangkok 10800, Thailand.ORCID https://orcid.org/0000-0002-1311-9030
Napachanok M SwainsonDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.ORCID https://orcid.org/0000-0001-6005-4316

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid and specific detection of N-terminal pro-B-type natriuretic peptide (NT-proBNP) is critical for improving heart-failure diagnosis. In this study, a single-chain variable fragment (scFv) recognizing NT-proBNP and incorporating a gold-assembling linker (C12AP) was engineered and affinity-matured to enhance binding performance for gold-surface biosensing applications. Structure-guided docking of the C12AP-NT-proBNP complex informed semirational mutagenesis targeting residues D110 in VH-CDR3 and Y174 in VL-CDR1. Twenty-two scFv variants were expressed in

Identifiers

PMID42255625
PMCPMC13234787

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.