Evidence map›Paper›PMID 42254993›Full record

ArticleJournal of biomolecular techniques : JBT2026

Direct RNA Sequencing of Dengue and Chikungunya Viruses with Ultra-Low Input and Custom Multiplexing.

Clément Tanvet, Chloé Baum, Olivia O'Connor, Chloé Bohers, Myrielle Dupont-Rouzeyrol, Marc Monot

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular techniques : JBT, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Clément TanvetPlate-Forme Technologique Biomics Université Paris Cité.
Chloé BaumPlate-Forme Technologique Biomics Université Paris Cité.
Olivia O'ConnorDengue and Arboviruses Research and Expertise Unit Institut Pasteur de Nouvelle-Calédonie.
Chloé BohersArboviruses and Insect Vectors Université Paris Cité.
Myrielle Dupont-RouzeyrolDengue and Arboviruses Research and Expertise Unit Institut Pasteur de Nouvelle-Calédonie.
Marc MonotPlate-Forme Technologique Biomics Université Paris Cité.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate viral genome characterization is essential for understanding viral dynamics. Oxford Nanopore Technologies (ONT) Direct RNA Sequencing provides detailed genomic data by directly reading RNA molecules, avoiding biases from reverse transcription and PCR. However, it requires high RNA inputs and lacks a multiplexing protocol, raising costs. This study explores Direct RNA Sequencing with minimal RNA inputs and multiplexing for arboviruses. We sequenced five strains, including dengue and chikungunya viruses, to test ultra-low RNA inputs and multiplexing. Results show successful sequencing and over 99% genome coverage with ultra-low inputs, highlighting the potential of Direct RNA Sequencing in genomic surveillance and the detection of RNA modifications. This method also shows promise for future application in sequencing patient serum samples without the need for viral cultivation.

Indexed as

Chikungunya virusDengue VirusRNA, ViralSequence Analysis, RNAChikungunya FeverDengueGenome, ViralHigh-Throughput Nucleotide SequencingHumansRNA, Viralchikungunya virusDengue virusDirect-RNA sequencingLow RNA inputMultiplexingNanopore

Identifiers

PMID42254993
PMCPMC13235691

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.