Evidence map›Paper›PMID 42254667›Full record

ArticlePeerJ2026

Comparative genomics of hospital-associated vancomycin-resistant

Chotiwit Sittiyuno, Mingkwan Yingkajorn, Sarunyou Chusri, Komwit Surachat, Arnon Chukamnerd, Amornrat Sianglum, Jiraphorn Nilsakul, Pimonsri Mittraparp-Arthorn, Wipawadee Sianglum

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chotiwit SittiyunoDivision of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Mingkwan YingkajornDepartment of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Sarunyou ChusriDivision of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Komwit SurachatDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Arnon ChukamnerdDivision of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Amornrat SianglumClinical Microbiology Laboratory, Department of Medical Technology and Clinical Pathology, Sunpasitthiprasong Hospital, Mueang, Ubon Ratchathani, Thailand.
Jiraphorn NilsakulClinical Microbiology Laboratory, Department of Medical Technology and Clinical Pathology, Sunpasitthiprasong Hospital, Mueang, Ubon Ratchathani, Thailand.
Pimonsri Mittraparp-ArthornDivision of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Wipawadee SianglumDivision of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Vancomycin-resistant Methods: Phenotypic testing included antimicrobial susceptibility testing (AST) and virulence factor testing (gelatinase, lipase, protease, hemolytic activity, and biofilm formation), which were performed using standard laboratory methods. Short-read whole-genome sequencing (WGS) was performed to determine sequence types (ST), identify antimicrobial resistance and virulence genes, and perform phylogenetic analysis using SNP alignments. Results: All isolates exhibited vancomycin and teicoplanin resistance, and γ-hemolysis was the predominant pattern (23 isolates). Biofilm formation was predominantly moderate to weak. Most isolates had multiple antimicrobial resistance and virulence-associated genes. Genotype and phenotype concordance were high for β-lactams, ciprofloxacin, and glycopeptides, whereas tetracycline (54.35%), streptomycin (45.65%), and gentamicin (45.65%) exhibited moderate concordance. Phylogenetic analysis revealed two major clades, Clade I (predominantly ST80) and Clade II (predominantly ST17). One isolate represented a novel ST, ST2331 (Clade I), which was identified from urine. Conclusion: VREfm isolates from the three hospitals showed multidrug-resistant profiles and carried various resistance and virulence-associated genes. Whole-genome sequencing revealed two major phylogenetic clades. Overall, genotypic findings corresponded well with the phenotypic resistance patterns, with a small number of isolates showing genotype-phenotype discrepancies. These results highlight the genomic diversity and clinical relevance of VREfm circulating in Thai tertiary hospitals.

Indexed as

Anti-Bacterial AgentsDrug Resistance, Multiple, BacterialEnterococcus faeciumGenomicsVancomycinVancomycin-Resistant EnterococciCross InfectionHumansMicrobial Sensitivity TestsPhylogenyTeicoplaninTertiary Care CentersThailandUrineWhole Genome SequencingAnti-Bacterial AgentsTeicoplaninVancomycinAntibiotic resistanceMultidrug resistanceVancomycin-resistant Enterococcus faeciumVirulence factorsWhole-genome sequencing

Identifiers

PMID42254667
PMCPMC13242186

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.