ArticleAnalytical chemistry2026
The Many Layers of Membrane Biophysics: Environment-Sensitive Fluorophores Report on Structural Organization of Biological Membranes at Various Depths.
Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Disorder in Order-Related Membrane Biophysical Parameters: An In-Depth Analysis of Di-4-ANEPPDHQ Generalized Polarization.The journal of physical chemistry letters · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular-order-related membrane biophysical properties contribute to the functional modulation of transmembrane proteins, and therefore, their changes due to a modified membrane composition in diseases associated with alterations in lipid levels may play important pathophysiological roles. In living cells, membrane biophysics can be examined with environment-sensitive fluorophores that describe the structural organization of biological bilayers from different aspects. Microviscosity-sensitive probes such as TMA-DPH characterize the degree of motional freedom, that is, fluidity, polarity-sensitive dyes including Laurdan or PY3174 report on the extent of water penetration, that is, hydration, whereas voltage-sensitive fluorophores such as di-8-ANEPPS quantify the nonrandom alignment of molecular dipoles, that is, the dipole potential. Given that all of the above properties are intrinsically linked to the arrangement of membrane constituents, such parameters are assumed to change in parallel with each other, and thus, the information gained by the environment-sensitive fluorophores is generally considered equivalent. In the current study, using fluorescence-based measurement techniques and manipulating membrane sterol levels with cyclodextrin-based complexes in living cells, we experimentally demonstrate incongruent changes in fluorescence properties of TMA-DPH, Laurdan, PY3174, and di-8-ANEPPS. Comparative MD simulations reveal that this can be due to the distinct membrane localization of the fluorophores. Our experimental and computational analyses reveal that the most commonly applied environment-sensitive fluorophores depict the structural organization of membranes at different depths and suggest that a comprehensive investigation of bilayer structure should include an appropriate combination of dyes, which would be required for a better understanding of membrane biophysics and its potential roles in disease pathogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.