Evidence map›Paper›PMID 42252666›Full record

ArticleBiotechnology and bioengineering2026

An Antibiotic-Free Cryptic Plasmid Platform for Modular Pathway Coupling in Probiotic Escherichia Coli Nissle 1917.

Xue Cai, Jie Cui, Lu Xiong, Jing-Hui Cao, Xin-Yan Lu, Jingjing Shi, Bo Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xue CaiHuadong Industry Technology Institute of Synthetic Biology, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Jie CuiHuadong Industry Technology Institute of Synthetic Biology, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Lu XiongZhejiang Key Laboratory of Bioorganic Synthesis, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Jing-Hui CaoZhejiang Key Laboratory of Bioorganic Synthesis, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Xin-Yan LuZhejiang Key Laboratory of Bioorganic Synthesis, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Jingjing ShiHuadong Industry Technology Institute of Synthetic Biology, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Bo ZhangState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Zhi-Qiang LiuHuadong Industry Technology Institute of Synthetic Biology, Zhejiang University of Technology, Hangzhou, Zhejiang, China.ORCID https://orcid.org/0000-0003-3259-6796
Yu-Guo ZhengHuadong Industry Technology Institute of Synthetic Biology, Zhejiang University of Technology, Hangzhou, Zhejiang, China.

Funding

Central Guidance on Local Science and Technology Development Fund of Zhejiang Province 2025ZY01010Huadong Medicine Joint Fund of the Zhejiang Provincial Natural Science Foundation of China LHDMZ25C050001
6 · The paper itself

Abstract

Escherichia coli Nissle 1917 (EcN) is a well-established probiotic chassis with an excellent safety profile; however, its application as an industrial microbial cell factory remains limited, largely due to genetic instability and antibiotic-dependent expression systems. In this study, we developed an antibiotic-free and genetically stable metabolic engineering strategy for vitamin B

Indexed as

Escherichia coliMetabolic EngineeringMetabolic Networks and PathwaysPlasmidsProbioticsAnti-Bacterial AgentsAnti-Bacterial Agentsantibiotic‐freecarbon redistributioncryptic plasmidEscherichia coli Nissle 1917genetic stability

Identifiers

PMID42252666
PMCPMC13521461

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.