Evidence map›Paper›PMID 42252416›Full record

ArticleBMC plant biology2026

Genome-wide identification of the MAPK gene family in peanut (Arachis hypogaea L.) and functional characterization of AhMPK3 and AhMPK18 in plant innate immunity.

Li Yang, Junjie Shi, Yuxin Yang, Sixian Hu, Heyi Lu, Hainan Jiang, Yao Cai, Like Wang, Ailing Ben, Tingli Liu and 1 more

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In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

11 authors.

Li Yang *The Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Junjie Shi *The Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Yuxin Yang *The Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Sixian HuThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Heyi LuThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Hainan JiangThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Yao CaiThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Like WangThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Ailing BenThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Tingli LiuThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China.
Yazhou BaoThe Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, China. yazhou_bao@163.com.

Funding

National Natural Science Foundation of China 32302309Natural Science Foundation of Jiangsu Province BK20230986
6 · The paper itself

Abstract

backgroundThe mitogen-activated protein kinase (MAPK) cascade is a fundamental signaling module that translates environmental stimuli into cellular responses in eukaryotes. Despite its importance, a comprehensive analysis of this family in the allotetraploid peanut (Arachis hypogaea L.) remains limited.

resultsIn this study, 32 AhMAPK family members (AhMPK1-AhMPK32) were identified in the peanut genome. These genes are non-uniformly distributed across 14 chromosomes and are characterized by a widespread absence of introns. Phylogenetic analysis categorized the AhMPK proteins into four distinct subgroups (Groups I, II, III, and Ⅳ), with high conservation in gene structures and protein motifs within each group. Promoter analysis revealed an abundance of cis-acting elements responsive to light, phytohormones, and abiotic stresses. Transcriptomic profiling across 19 tissues and multiple stress conditions showed that while most AhMPK genes are constitutively expressed, AhMPK3 and AhMPK18 maintain high transcript abundance and are significantly induced by various environmental stimuli. Functional characterization through Agrobacterium-mediated transient and stable expression demonstrated that AhMPK3 and AhMPK18 positively regulate plant basal immunity. Overexpression of these genes significantly enhanced PAMP-induced reactive oxygen species (ROS) bursts, up-regulated the defense marker gene FRK1, and conferred increased resistance to Pseudomonas syringae pv. tomato (Pst) DC3000.

conclusionsCollectively, our results provide a systematic genomic framework for the peanut AhMAPK family and identify AhMPK3 and AhMPK18 as critical candidates for the genetic improvement of disease resistance and stress resilience in peanut.

Indexed as

ArachisMitogen-Activated Protein KinasesPlant ImmunityPlant ProteinsDisease ResistanceGene Expression ProfilingGene Expression Regulation, PlantGenome, PlantImmunity, InnateMultigene FamilyPhylogenyPlant DiseasesMitogen-Activated Protein KinasesPlant ProteinsAbiotic stressAhMAPK cascadeExpression profilingGenome-wide identificationPeanutPlant innate immunity

Identifiers

PMID42252416
PMCPMC13464904

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