Evidence map›Paper›PMID 42251147›Full record

ArticleScientific reports2026

Extraction-dependent phenolic shifts drive antioxidant and multi-enzyme inhibitory activities of Onosma rechingeri methanolic extracts.

Mamdouh Alshammari, Cengiz Sarikurkcu, Fevzi Bardakci, Mousa Alreshidi, Mohd Adnan, Arif Jamal Siddiqui, Bektas Tepe

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Mamdouh AlshammariDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.ORCID http://orcid.org/0009-0007-7119-5043
Cengiz SarikurkcuFaculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar, TR-03100, Turkey.ORCID http://orcid.org/0000-0001-5094-2520
Fevzi BardakciDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia. fevzi.bardakci@gmail.com.ORCID http://orcid.org/0000-0003-3377-2763
Mousa AlreshidiDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.ORCID http://orcid.org/0000-0002-3763-9698
Mohd AdnanDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.ORCID http://orcid.org/0000-0002-7080-6822
Arif Jamal SiddiquiDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.ORCID http://orcid.org/0000-0002-6236-0920
Bektas TepeDepartment of Molecular Biology and Genetics, Faculty of Science, Kilis 7 Aralik University, Kilis, TR-79000, Turkey. bektastepe@kilis.edu.tr.ORCID http://orcid.org/0000-0001-8982-5188

Funding

Scientific Research Deanship at the University of Ha'il - Saudi Arabia RG-24 089
6 · The paper itself

Abstract

The bioprospecting of Onosma species for multifunctional phenolics is gaining momentum, yet the impact of extraction methods on bioactivity is underexplored. This study compared methanolic extracts of O. rechingeri obtained via ultrasound-assisted extraction (UAE-ME), maceration (MAC-ME), and Soxhlet (SOE-ME). Total phenolic and flavonoid content was highest in SOE-ME. Luteolin-7-glucoside and hesperidin were abundant in MAC-ME, while SOE-ME was richest in rosmarinic acid. SOE-ME showed superior electron-transfer antioxidant activity, while metal chelation favored UAE-ME and MAC-ME. Enzyme inhibition varied by extraction: MAC-ME was most effective against AChE and α-amylase, SOE-ME against tyrosinase. Correlation analysis revealed distinct compound-activity associations: tyrosinase aligned with electron transfer, AChE and α-amylase with chelation. Docking suggested luteolin-7-glucoside as a strong α-amylase binder (-9.09 kcal/mol) and rosmarinic acid as a promising in silico binder of AChE and TYRP1. Overall, extraction strategy significantly alters chemical profiles and bioactivity, but these findings should be interpreted as preliminary because they are based on crude extracts, in vitro assays, correlation analysis with a limited sample set, and docking predictions; therefore, they highlight the need for targeted fractionation and further biological validation.

Indexed as

AntioxidantsEnzyme InhibitorsPhenolsPlant ExtractsAcetylcholinesterasealpha-AmylasesCinnamatesFlavonoidsLuteolinMethanolMolecular Docking SimulationMonophenol MonooxygenaseRosmarinic AcidAcetylcholinesterasealpha-AmylasesAntioxidantsCinnamatesEnzyme InhibitorsFlavonoidsLuteolinMethanolMonophenol MonooxygenasePhenolsPlant ExtractsRosmarinic AcidAntioxidant activityEnzyme inhibitionIn silico dockingLC-ESI-MS/MS profilingOnosma rechingeriUltrasound-assisted extraction

Identifiers

PMID42251147
PMCPMC13482576

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.