Protocol for isolation of nuclei from murine cardiac tissue for single-nucleus multiomic sequencing.

Ioanni Veras, Olav Søvik Eken, Finn Olav Levy, Arne Olav Melleby, Jan Magnus Aronsen

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ioanni VerasDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. Electronic address: ioanni.veras@medisin.uio.no.

Olav Søvik EkenDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Finn Olav LevyDepartment of Pharmacology, Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Pharmacology, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway.

Arne Olav MellebyDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Jan Magnus AronsenDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway; Department of Pharmacology, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway. Electronic address: j.m.aronsen@medisin.uio.no.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pathological cardiac remodeling involves cell-type-specific regulatory changes that require integrative analysis across all cardiac cell populations. Here, we present a protocol for isolating single nuclei from fresh-frozen murine cardiac ventricular tissue to enable the integrated analysis of gene expression and chromatin accessibility. We describe steps for mechanical homogenization, sequential filtration, sucrose cushion purification, and fluorescence-activated nuclei sorting (FANS). This protocol enables multiomic analysis across various cardiac cell types and supports epigenomic studies of gene regulation.

Indexed as

Cell NucleusMyocardiumSingle-Cell AnalysisAnimalsMiceMultiomicsGenomicsMolecular BiologySingle Cell

Identifiers

PMID42250224

DOI10.1016/j.xpro.2026.104615

PMCPMC13260199

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.