Protocol for a murine transient middle cerebral artery occlusion model with local intra-arterial drug delivery.

Jordan J Lee, Michael P Murphy, Thomas Krieg

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jordan J LeeDepartment of Medicine, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge CB2 0QQ, UK; MRC Mitochondrial Biology Unit, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: jjl71@cam.ac.uk.

Michael P MurphyDepartment of Medicine, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge CB2 0QQ, UK; MRC Mitochondrial Biology Unit, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.

Thomas KriegDepartment of Medicine, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge CB2 0QQ, UK. Electronic address: tk382@medschl.cam.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for a murine transient middle cerebral artery occlusion (tMCAO) model incorporating an intra-arterial catheter for local drug delivery during reperfusion. We describe steps for pre-operative preparation, surgical procedure, and post-operative care. We detail procedures for the insertion of a microcatheter for targeted drug delivery during reperfusion to mimic clinical mechanical thrombectomy. This approach enables investigation of adjunct therapies for ischemia-reperfusion injury. For complete details on the use and execution of this protocol, please refer to Lee et al.

Indexed as

Drug Delivery SystemsInfarction, Middle Cerebral ArteryAnimalsDisease Models, AnimalMiceMetabolismModel OrganismsNeuroscience

Identifiers

PMID42250223

DOI10.1016/j.xpro.2026.104618

PMCPMC13260088

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.