ArticleMolecular biology reports2026
Breaking clonal barrier in citronella (Cymbopogon winterianus Jowitt) by polycross breeding and SSR-based identification of pollen donor.
Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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- SSR-based paternity analysis in citronella polycross breeding: progress and remaining challenges.Molecular biology reports · 2026Review
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Abstract
backgroundLimited genetic and genomic resources in citronella necessitate the use of novel breeding methods to develop advanced breeding material that taps into genetic variability.
objectiveIdentify superior and highly variable half sibs besides identifying their pollen donors, to be used in citronella breeding programs.
methodsIn this study, we used four commercial citronella cultivars (CIM-Bio-13, CIM-Jeeva, Medini and Jal Pallavi) as parents in a polycross breeding scheme. This resulted in 60 half-sib progenies. We evaluated herb yield, essential oil content and various biochemical components across two harvests.
resultsFrom this evaluation, eleven superior half-sibs were identified. Notably, half-sib progeny B25 6-10 consistently outperformed others and showed the greatest divergence in both harvests. Principal component analysis accounted for 60-70% of observed variability with two components, highlighting the diversity within the progenies. SSR markers confirmed the highest amplification from Cymbopogon flexuous (54.26%), followed by C. winterianus (42.85%) and C. citratus (41.17%). Genetic diversity metrics confirmed moderate polymorphism and marker informativeness. SSR-based UPGMA clustering corroborated morphological differentiation, and unique pollen donors were identified for 5 of 11 half-sib progenies.
conclusionThe identified variable progenies are valuable resources for further genetic studies and potential population improvement. Marker-based paternity results demonstrate the value of this approach for precise selection in citronella breeding.
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