Evidence map›Paper›PMID 42250117›Full record

ArticleMarine biotechnology (New York, N.Y.)2026

Unveiling Complete Genome of Streptococcus agalactiae from Malaysian Aquaculture: A Closer Look at Molecular Characteristics and Phylogenomic.

Helmi Husaini Zainal Fithri, Nurul Hidayah Samsulrizal, Nur-Nazifah Mansor, Nurasyikin Hamzah, Noor Hanis Abu Halim, Mohd Syafiq Mohammad Ridzuan, Azila Abdullah, Nur Amalin Sofea Abdul Rahim, Azzmer Azzar Abdul Hamid

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Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Helmi Husaini Zainal FithriDepartment of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia.
Nurul Hidayah Samsulrizal *Department of Plant Science, Kuliyyah of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia. hidayahsamsulrizal@iium.edu.my.
Nur-Nazifah MansorDepartment of Marine Science, Kuliyyah of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia.
Nurasyikin HamzahDepartment of Chemistry, Kuliyyah of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia.
Noor Hanis Abu HalimNational Fish Health Research Centre (NaFisH), Fisheries Research Institute, Batu Maung, Penang, 11960, Malaysia.
Mohd Syafiq Mohammad RidzuanNational Fish Health Research Centre (NaFisH), Fisheries Research Institute, Batu Maung, Penang, 11960, Malaysia.
Azila AbdullahNational Fish Health Research Centre (NaFisH), Fisheries Research Institute, Batu Maung, Penang, 11960, Malaysia.
Nur Amalin Sofea Abdul RahimDepartment of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia.
Azzmer Azzar Abdul Hamid *Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia. azzmer@iium.edu.my.

Funding

12th Malaysia Plan budget: R&D of Fish Health Programs in Aquaculture P21300040170502
6 · The paper itself

Abstract

Streptococcus agalactiae (Group B Streptococcus, GBS) is a significant pathogen in aquaculture worldwide and is responsible for high mortality in farmed fish. Despite its regional impact, complete genome data from Malaysian isolates remain scarce. In this study, we report the first complete genome of a Malaysian S. agalactiae isolate, SA2BKE, derived from infected tilapia. Using Oxford Nanopore long-read sequencing, we assembled a 2.03 Mb circular complete genome of S. agalactiae. The functional annotation revealed 1,970 protein-coding genes and 108 RNA genes. Several antimicrobial resistance genes, including tet(M), mreA, and mprF, are associated with resistance to tetracyclines, macrolides, and peptides, respectively. Notably, there are 15 virulence-associated proteins involved in cell wall/membrane/envelope biogenesis. Multilocus sequence typing (MLST) identified SA2BKE as sequence type ST283, which has the potential to infect both fish and humans. Comparative phylogenomic analysis revealed 215 global strains positioned SA2BKE within a clade of other ST283 isolates from Asia and South America, suggesting potential transregional transmission. Pan-genome analysis identified 555 core genes shared among the analysed genomes, highlighting substantial genomic diversity within the species. Additionally, 14 mobile genetic element-associated regions were detected in SA2BKE, indicating potential genome plasticity and horizontal gene transfer events. These findings expand the genomic reference data for S. agalactiae isolates from Malaysia, contributing to regional surveillance efforts across Southeast Asia and supporting integrated disease management strategies in aquaculture.

Indexed as

Fish DiseasesGenome, BacterialStreptococcal InfectionsStreptococcus agalactiaeTilapiaAnimalsAquacultureDrug Resistance, BacterialMalaysiaMultilocus Sequence TypingPhylogenyWhole Genome SequencingMultilocus sequence typing (MLST)PhylogenomicsStreptococcus agalactiaeWhole-genome sequencing (WGS)

Identifiers

PMID42250117

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.