Evidence map›Paper›PMID 42250006›Full record

ArticlePlant cell reports2026

Identification of the NBS-LRR gene family and AkNBS-LRR43 and 100 functional analysis under biotic stress.

Haoliang Shi, Dandan Gao, Min Yang, Ying Qi, Jianwei Guo, Penghua Gao, Feiyan Huang, Jiani Liu, Jianrong Zhao, Lifang Li and 1 more

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Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Haoliang ShiYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Dandan GaoYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Min YangYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Ying QiYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Jianwei GuoYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Penghua GaoYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Feiyan HuangYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Jiani LiuYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Jianrong ZhaoYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China.
Lifang LiYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China. csqsmile@126.com.
Lei YuYunnan Key Laboratory of Konjac Biology, College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming, 650214, China. yulei0425@163.com.

Funding

Yunnan Provincial Science and Technology Department 202401AU070020, 202449CE340009, 202503AP140005
6 · The paper itself

Abstract

key messageAkNBS-LRR43 and AkNBS-LRR100 positively regulate immunity in Amorphophallus konjac against Pectobacterium carotovorum subsp. carotovorum by enhancing antioxidant activity and reducing oxidative damage. Amorphophallus konjac, an economically important Araceae species rich in konjac glucomannan (KGM), faces production losses from biotic/abiotic stresses. Nucleotide-binding-site leucine-rich repeat (NBS-LRR) genes, crucial for plant immunity, remain understudied in A. konjac. Here, we comprehensively analyzed 146 AkNBS-LRR genes, characterizing their structure, chromosomal distribution, phylogeny, cis-elements and expression patterns. Furthermore, two candidate genes (AkNBS-LRR43/100) were functionally validated. The AkNBS-LRRs showed chromosomal preference (chr1/2/3/5/9/11) and clustered into five phylogenetic subclasses (CNL_E predominant). These genes harbored abundant cis-elements associated with growth and stress responses. Functional annotation implicated their roles in signal transduction, development, and biotic/abiotic stress responses. Transcriptome profiling revealed pathogen-responsive differential expression patterns. RT-qPCR revealed differential AkNBS-LRR expression during Pectobacterium carotovorum subsp. carotovorum (Pcc) infection, with AkNBS-LRR43/100 showing pronounced upregulation. Subcellular localization confirmed their nuclear targeting. Transgenic A. konjac plants transiently overexpressing AkNBS-LRR43/100 showed significantly reduced lesion expansion under pathogen infection compared to controls. Concurrently, these transgenic plants exhibited increased activities of peroxidase (POD) and superoxide dismutase (SOD), along with decreased malondialdehyde (MDA) content, which may lead to enhanced reactive oxygen species (ROS) scavenging capacity and consequently to reduced disease symptom development. These findings suggest that AkNBS-LRR43/100 may function as positive regulators in A. konjac disease resistance by enhancing antioxidant capacity.

Indexed as

AmorphophallusMultigene FamilyPlant ProteinsStress, PhysiologicalBinding SitesDisease ResistanceGene Expression Regulation, PlantLeucine-Rich Repeat ProteinsMalondialdehydePectobacterium carotovorumPeroxidasePhylogenyPlant DiseasesLeucine-Rich Repeat ProteinsMalondialdehydePeroxidasePlant ProteinsAbiotic stressAmorphophallus konjacBioinformaticsBiotic stressNBS-LRR gene family

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.