Evidence map›Paper›PMID 42249450›Full record

ArticleCell communication and signaling : CCS2026

Protein arginine methyltransferase 5 orchestrates the homeostasis and reconstitution of intestinal stem cell niche via balancing urea cycling.

Nan Wang, Xi Wang, Qihang Lian, Yikun Luo, Hefei Tian, Banghui Liu, Yujun Huang, Zhenni Xu, Xudan Lei, Lingxiao Huang and 1 more

Abstract read
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In one paragraph

Article in Cell communication and signaling : CCS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nan Wang *Precision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Xi Wang *Precision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Qihang Lian *Precision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Yikun LuoPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Hefei TianPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Banghui LiuPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Yujun HuangPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Zhenni XuPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Xudan LeiPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Lingxiao HuangPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Dengqun LiuPrecision Radiation in Oncology Key Laboratory of Sichuan Province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China. dengqunliu@uestc.edu.cn.

Funding

National Natural Science Foundation of China 82570643,81874255,81673089Science and Technology Department of Sichuan Province 2025NSFJQ0058
6 · The paper itself

Abstract

backgroundProtein arginine methyltransferase 5 (PRMT5) is highly expressed in many cancers and is a potential therapeutic target. It is also expressed in the small intestine, suggesting a role in intestinal health. This study explores PRMT5's function in both normal physiology and radiation-induced intestinal injury (RIII), focusing on its effects on intestinal stem cells (ISCs) and their niche.

methodsWe examined PRMT5 expression in healthy and radiation-damaged intestines and treated mice and organoids with AMI-1, a PRMT5 inhibitor. Epithelial lineage composition, ISC proliferation, inducible nitric oxide synthase (iNOS) levels, and organoid activity were assessed. The impact of PRMT5 deficiency on ISC function was studied in vitro, and RNA-Seq and qRT-PCR were used to explore its effects on the urea cycle.

resultsPRMT5 was highly expressed in intestinal crypts. AMI-1 treatment reduced small intestine length, altered epithelial morphology, and increased secretory cells. In healthy intestines, PRMT5 inhibition enhanced Olfm4 + ISCs and induced iNOS expression. After radiation, PRMT5 deficiency inhibited ISC proliferation and caused Paneth cell acidification in the ISC niche. Organoids showed reduced vitality. PRMT5 deficiency disrupted the urea cycle, upregulated iNOS, increased NO production, and elevated lipid and ROS levels, impairing ISC homeostasis.

conclusionsPRMT5 is critical for maintaining intestinal homeostasis and regeneration. Its deficiency disrupts ISC niche function, highlighting PRMT5 as a potential target for treating intestinal disorders.

Indexed as

HomeostasisIntestinesProtein-Arginine N-MethyltransferasesStem Cell NicheUreaAnimalsCell ProliferationIntestinal MucosaIntestine, SmallMiceMice, Inbred C57BLNitric Oxide Synthase Type IIOrganoidsStem CellsNitric Oxide Synthase Type IIPrmt5 protein, mouseProtein-Arginine N-MethyltransferasesUreaiNOSIntestinal stem cellsPaneth cellPRMT5Urea cycling

Identifiers

PMID42249450
PMCPMC13295621

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.