Evidence map›Paper›PMID 42247049›Full record

ArticleVeterinary research communications2026

Sustainable valorization of citrus pomace waste: biochar production and in vitro bioactivities.

Cristina Spedale, Vincenzo Chiofalo, Matteo Dell'Anno, Sara Frazzini, Marco Puglia, Simone Pedrazzi, Alessandra Moscatelli, Elisabetta Onelli, Luciana Rossi

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Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cristina SpedaleDepartment of Veterinary Sciences, University of Messina, Polo Universitario Annunziata, Messina, 98168, Italy.
Vincenzo ChiofaloDepartment of Veterinary Sciences, University of Messina, Polo Universitario Annunziata, Messina, 98168, Italy.
Matteo Dell'AnnoDepartment of Veterinary Sciences, University of Messina, Polo Universitario Annunziata, Messina, 98168, Italy. matteo.dellanno@unime.it.ORCID http://orcid.org/0000-0002-2171-864X
Sara FrazziniDepartment of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, 26900, Italy.
Marco PugliaDepartment of Engineering "Enzo Ferrari", University of Modena and Reggio Emilia, Modena, Italy.
Simone PedrazziDepartment of Engineering "Enzo Ferrari", University of Modena and Reggio Emilia, Modena, Italy.
Alessandra MoscatelliDepartment of Biosciences, University of Milan, Milan, Italy.
Elisabetta OnelliDepartment of Biosciences, University of Milan, Milan, Italy.
Luciana RossiDepartment of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, 26900, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In recent years, biochar, a carbon-rich material, has gained increasing interest in zootechnical applications due to its potential antioxidant and antimicrobial properties. Sicily is the leading citrus-producing region in Italy, generating large amounts of agro-industrial waste, which can be converted into biochar, representing an innovative approach. This study investigated the chemical composition, mineral content, morphology, enzymatic digestibility, antioxidant and antimicrobial activity of biochar obtained by pyrolysis of whole lemon pomace (WB) and depectinized lemon pomace (DB). WB and DB biomasses were pyrolyzed at 600 °C for 60 min. Samples were analyzed for bromatological and mineral composition; morphological characterization was evaluated through SEM, and antioxidant analysis through ABTS assay for biochar extracts. Antimicrobial activity was evaluated using a microdilution method. WB extract exhibited higher antioxidant and antimicrobial activity against E. coli compared to DB extract, with an antioxidant activity of 306.79 ± 22.23 µg of TE/g (p < 0.001). Potential undesirable elements were very low in both samples. Particularly, copper was lower in DB, while zinc was higher in DB (p < 0.0001). SEM analysis revealed macropores and mesopores attributable to xylem vessels and fibers in both biochar samples: WB sample exhibited values up to 35 μm and down to 15 nm; DB revealed pores from 20 μm to 200 nm. These findings highlight the value of citrus pomace biochar extracts for their potential antioxidant and antimicrobial properties against E. coli, demanding further in vivo investigations to confirm its efficacy and benefits in livestock.

Indexed as

Anti-Infective AgentsAntioxidantsCharcoalCitrusAnti-Bacterial AgentsEscherichia coliIndustrial WastePyrolysisAnti-Bacterial AgentsAnti-Infective AgentsAntioxidantsbiocharCharcoalIndustrial WasteAnimal healthAntimicrobial activityAntioxidant activityLemon by-productsPyrolysis

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.