ArticlePest management science2026
Fusion dsRNA targeting ecdysteroid signaling provides efficient and selective RNAi-based control of spider mites.
Article in Pest management science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundTetranychus urticae is a major agricultural pest mite whose rapid evolution of pesticide resistance necessitates innovative control strategies. RNA interference (RNAi) offers a promising alternative to chemical pesticides for its high specificity, efficiency, and environmental safety. Ecdysteroid signaling genes, which regulate molting and development in arthropods, represent potential RNAi targets for inducing lethal molting defects. This study investigated the roles of ecdysteroid signaling pathway genes in T. urticae molting and evaluated the potential of fusion double-stranded RNA (dsRNA) constructs targeting multiple genes to enhance RNAi efficacy.
resultsThe expression profiling revealed that TuE74, TuECR, TuHR4, TuE93, and TuHR3 were highly expressed during the molting stage of T. urticae. RNAi-mediated silencing of these genes, particularly TuECR, TuHR3, TuHR4, and TuE74, caused distinct molting defects and high mortality. Fusion dsRNAs targeting combinations of these genes (ECR-HR3-HR4 or ECR-HR3-E74) achieved stronger and more coordinated gene silencing, lower median lethal concentration (LC
conclusionThe present study demonstrates that multi-target fusion dsRNA can substantially enhance RNAi efficacy, broaden control spectra across pest mite species, and maintain environmental safety. These results provide a molecular basis and applied framework for the development of next-generation RNAi-based acaricides for sustainable spider mite management. © 2026 Society of Chemical Industry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.