Evidence map›Paper›PMID 42246250›Full record

ArticleJournal of proteome research2026

Development and Validation of a Streamlined Workflow for Proteomic Analysis of Proteins and Post-translational Modifications from Dried Blood.

Matthew W Foster, Youwei Chen, Marlene J Violette, Sarah M Shapley, Michael T Forrester, J Scott Mellors, Brett S Phinney, Robert S Plumb, J Will Thompson, Timothy J McMahon

Abstract readValidation Study
In one paragraph

Article in Journal of proteome research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Matthew W FosterDivision of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, United States.ORCID 0000-0003-0212-2346
Youwei ChenDivision of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, United States.
Marlene J VioletteProteomics and Metabolomics Core Facility, Duke University School of Medicine, Durham, North Carolina 27701, United States.
Sarah M ShapleyProteomics and Metabolomics Core Facility, Duke University School of Medicine, Durham, North Carolina 27701, United States.
Michael T ForresterDivision of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, United States.ORCID 0009-0007-4665-4164
J Scott Mellors908 Devices Inc., Boston, Massachusetts 02210, United States.
Brett S PhinneyProteomics Core, University of California Davis, Davis, California 95616, United States.ORCID 0000-0003-3870-3302
Robert S PlumbWaters Corporation, Milford, Massachusetts 01757, United States.ORCID 0000-0002-1380-9285
J Will Thompson908 Devices Inc., Boston, Massachusetts 02210, United States.
Timothy J McMahonDivision of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, United States.ORCID 0000-0002-3404-3223

Funding

Red blood cell ATP export and transfusion in sepsisR01HL161071 · NHLBI · DUKE UNIVERSITY · PI Allan Doctor, TIMOTHY J MCMAHON · 2023 to 2026
$2.7M
Multiomic, mass spectrometry-based analysis of dried blood for deep phenotyping of sepsisR33GM146142 · NIGMS · DUKE UNIVERSITY · PI FOSTER, MATTHEW WOLF, MCMAHON, TIMOTHY J · 2024 to 2025
$1.4M
Multiomic, mass spectrometry-based analysis of dried blood for deep phenotyping of sepsisR21GM146142 · NIGMS · DUKE UNIVERSITY · PI FOSTER, MATTHEW WOLF, MCMAHON, TIMOTHY J · 2022 to 2023
$439k
NHLBI NIH HHS R01 HL161071NIGMS NIH HHS R21 GM146142NIGMS NIH HHS R33 GM146142
6 · The paper itself

Abstract

It is increasingly recognized that the 'omic analysis of whole blood has applications for precision medicine and disease phenotyping. Despite this realization, whole blood is generally viewed as a challenging analytical matrix in comparison to plasma or serum. Moreover, proteomic analyses of whole blood have almost exclusively focused on (non)targeted analyses of protein abundances and much less on post-translational modifications (PTMs). Here, we developed a streamlined workflow for processing 20 microliters of venous blood collected by volumetric absorptive microsampling that incorporates serial trypsinization and

Indexed as

Blood ProteinsDried Blood Spot TestingProtein Processing, Post-TranslationalProteomeProteomicsChromatography, LiquidGlycopeptidesHumansLiquid Chromatography-Mass SpectrometryPhosphopeptidesTandem Mass SpectrometryWorkflowBlood ProteinsGlycopeptidesPhosphopeptidesProteomedata-independent acquisitionHILICIMACmicroflow liquid chromatographyMitra deviceMOFAOrbitrap Astralstepped collision energyVAMS

Identifiers

PMID42246250
PMCPMC13387486

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.