Cochlear isolation in neonatal mice for

Introduction: Methods: We propose an optimized stepwise technique for cochlear isolation based on dissection of skull bones along specific anatomical skull lines-the spheno-occipital, spheno-petrosal, and petro-occipital synchondroses, along with the petrosquamosal suture. This approach utilizes targeted dissection along natural anatomical skull lines to carefully separate skull bones, reducing excess tissue and unnecessary manipulation of the cochlea. Sample preservation was assessed through immunofluorescence staining for hair cells (phalloidin) and spiral ganglion neurons (β-III tubulin) in both Results: The technique significantly reduced superfluous tissue adherence, thereby facilitating subsequent processing of the cochlear surface. Intact cochleae were consistently obtained with preserved auditory capsules and the structure of the membranous labyrinth. Immunofluorescence analysis confirmed the preservation of inner ear samples obtained for creating Conclusion: Cochlear isolation is a technically challenging procedure that requires both practiced skill and careful execution. A key feature of our protocol lies in the emphasis on specific anatomical landmarks, which facilitates a clearer understanding of the cochlear isolation process and prevents actions that would compromise the integrity of the cochlea. This is important for researchers who are developing the skills necessary to create