ArticleZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences2026
[Application of puncture tissue rinse solution in the detection of
Article in Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectivesPrecision targeted therapy for non-small cell lung cancer (NSCLC) relies on molecular classification based on gene mutations. Detection of driver gene mutations, particularly epidermal growth factor receptor (EGFR) mutations, is crucial for treatment decision-making in locally advanced and metastatic NSCLC. Currently, clinical sources of nucleic acids include formalin-fixed paraffin-embedded (FFPE) tumor tissues, cytological specimens from centrifuged pleural effusion, blood, and circulating free DNA in body fluids such as pleural effusion supernatant. This study aims to extract nucleic acids from the double-distilled water rinse solution of lung biopsy specimens for gene mutation detection, to evaluate the feasibility of this nucleic acid source, and to analyze the concordance between its detection results and those obtained from tissue-based next-generation sequencing (NGS), thereby providing a basis for subsequent pathological diagnosis and treatment.
methodsFemale patients clinically diagnosed with NSCLC and admitted to the Department of Oncology, the Second Xiangya Hospital of Central South University were included. CT-guided percutaneous lung biopsy was performed. Biopsy tissues were processed into paraffin-embedded sections for NGS testing. Meanwhile, DNA was extracted from the double-distilled water rinse solution of biopsy tissues, and EGFR gene mutations were detected using the super amplification refractory mutation system-polymerase chain reaction (Super-ARMS). Samples with remaining DNA meeting quality control standards were further subjected to NGS. The concordance between gene mutation results from the rinse solution and those from corresponding FFPE tissue samples was analyzed. In addition, DNA concentrations from percutaneous biopsy specimens and from the rinse solution of the puncture needle were measured and compared.
resultsAmong 29 NSCLC patients, Super-ARMS of the biopsy tissue rinse solution detected 19
conclusionsTDNA extracted from the double-distilled water rinse solution of CT-guided percutaneous lung biopsy specimens can serve as a rapid and efficient nucleic acid source for NSCLC genotyping, enabling earlier support for clinical diagnosis and treatment.
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