Evidence map›Paper›PMID 42243677›Full record

ArticleBMC plant biology2026

Fine mapping and transcriptomic analysis reveal BnaMMS, a novel locus regulating multi-main-stem phenotype via auxin pathway in rapeseed.

Rui Xia, Jie Liang, Pengfei Wang, Wanli Zhang, Wen Wang, Qing Li, Lianjin Zeng, Xue Li, Haiyan Zhang, Chengcheng Si and 2 more

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Rui XiaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Jie LiangSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Pengfei WangNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Wanli ZhangSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Wen WangSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Qing LiSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Lianjin ZengSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Xue LiYazhouwan National Laboratory, Sanya, 572025, China.
Haiyan ZhangSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China. 18140526182@163.com.
Chengcheng SiSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
Dengfeng HongNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Xianming ZhouSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China. xm.zhou@hainanu.edu.cn.

Funding

Hainan Provincial Natural Science Foundation of China 323QN192National Natural Science Foundation of China 32501930Research Initiation Fund of Hainan University KYQD(ZR)-22103
6 · The paper itself

Abstract

The multi-main-stem (MMS) trait presents considerable potential for improving plant architecture and increasing yield in rapeseed (Brassica napus L.). However, the molecular mechanism underlying MMS formation remains poorly understood. In this study, we characterized a dominant MMS material, 1030R. Genetic analysis indicated that the MMS phenotype is controlled by a single dominant gene, designated BnaMMS. Utilizing BSA-seq combined with fine mapping, BnaMMS was localized to a 70 kb physical interval on chromosome A6, representing a novel genetic locus for MMS regulation. This region contains 17 annotated genes. Sequence analysis revealed multiple SNP and Indel variations between the parental lines. Transcriptome profiling further demonstrated that, among these candidates, two genes (BnaA06G0092000ZS and BnaA06G0092200ZS) were differentially expressed between near-isogenic lines (NILs) displaying MMS and single-main-stem (SMS) phenotypes. Mechanistic investigations suggested that, with no significant changes observed in the expression of core WUS-CLV pathway genes in our transcriptome data, BnaMMS likely regulates MMS formation by disrupting auxin biosynthesis and distribution. Our work identifies a novel genetic locus controlling MMS and provides valuable genetic resources and a theoretical framework for elucidating the molecular mechanisms of stem development and for breeding high-yielding rapeseed varieties with optimized plant architecture.

Indexed as

Brassica napusIndoleacetic AcidsPlant StemsChromosome MappingGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantPhenotypeTranscriptomeIndoleacetic AcidsAuxinBnaMMSBrassica napusBSA-seqMulti-main-stemTranscriptome

Identifiers

PMID42243677
PMCPMC13455139

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.