Evidence map›Paper›PMID 42243664›Full record

ArticleBMC genomic data2026

Draft genome sequence of Staphylococcus saprophyticus strain 3M isolated from milk of cows with subclinical mastitis in Uganda.

Reagan Muhwezi, G R Neel, Emmanuel Eilu, Vidya Sankarapandian, Ambrose Shabohurira, Musoba Abubakar, Theophilus Pius, Priscilla Peter Dilli, Naheem Adekilekun Tijani, Danladi Makeri

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Article in BMC genomic data, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Reagan MuhweziDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda. muhwezi@kiu.ac.ug.
G R NeelDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Emmanuel EiluInstitute of Allied Health Sciences, Clarke International University, Kampala, Uganda.
Vidya SankarapandianDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Ambrose ShabohuriraDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Musoba AbubakarInstitute of Biomedical Research, Kampala International University Western Campus, Ishaka, Uganda.
Theophilus PiusDepartment of Medical Laboratory Science, Kampala International University Western Campus, Ishaka, Uganda.
Priscilla Peter DilliDepartment of Public Health, Kampala International University Western Campus, Ishaka, Uganda.
Naheem Adekilekun TijaniDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Danladi MakeriDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study presents the draft genome sequence of Staphylococcus saprophyticus strain 3M isolated from a cow with subclinical mastitis (SCM) in Kiruhura district, Uganda and explores the isolates resistance and virulence potential. DATA DESCRIPTION: The assembled genome size was 2,791,445 bp with a 33% GC, 13 Contigs, Contig N50 2,589,240, Contig L50 1 and 2,791,440x Genome coverage. Check M analysis revealed 68.25% completeness. Average Nucleotide Identity (ANI) analysis revealed Staphylococcus saprophyticus subsp. saprophyticus ATCC 15,305 = NCTC 7292(GCA_006094355.1) as the closest type strain with an ANI of 99.52%, 87.49% assembly coverage, 93.95% type assembly coverage, confirming species-level identity and close relatedness. Screening with the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) revealed several genes conferring resistance to different classes of antibiotics: antibiotic tet (K)- Tetracyclines, norA, YkkCD, ANT (6)-I, str, and S12p- Aminoglycosides. And also, Virulence factors included: Agr- biofilm and toxins release, TcaR- Protein A, MprF- resist the host's innate immune system (specifically, antimicrobial peptides). Other genome characteristics are presented in Table 1 below. Table 1 Data availability, genome statistics and features of strain 3M Parameter Value Genes 2,885 CDSs 2,801 rRNAs 7, 6, 6 (5 S, 16 S, 23 S) tRNAs 61 ncRNAs 4.

Indexed as

Genome, BacterialMastitis, BovineMilkStaphylococcal InfectionsStaphylococcusAnimalsCattleDrug Resistance, BacterialFemaleUgandaVirulence FactorsVirulence FactorsAntimicrobial-resistant genesBacterial genomicsGenomic annotationMinIon MK1B sequencingSubclinical mastitis

Identifiers

PMID42243664
PMCPMC13459452

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.