ArticleWorld journal of microbiology & biotechnology2026
Loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) for detection of wine microorganisms.
Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Wine is undoubtedly one of the oldest and most celebrated beverages across cultures. It is primarily crafted from grapes through a fermentation process where yeast transforms sugars into alcohol. The presence of unwanted microorganisms can cause spoilage, so early detection of microorganisms is crucial. The previously reported conventional methods and molecular methods have so many drawbacks they are time consuming and expensive to be used in wine industries. Therefore, this study presents a novel broad-range loop-mediated isothermal amplification coupled with lateral flow dipstick (LAMP-LFD) assay for the rapid detection of wine-related microorganisms. The novelty of this work lies in the design and modification broad-range LAMP primer sets, labelled with biotin and fluorescein isothiocyanate, enabling simultaneous detection of wine spoilage microorganisms, including acetic acid bacteria and Brettanomyces fungi, as well as beneficial wine fermentation microorganisms such as lactic acid bacteria and Saccharomyces cerevisiae, with immediate visualisation of amplification products on the LFD strip. The results illustrate that the designed primers were specific for wine-related microorganisms at optimised conditions in the LAMP-LFD. The newly developed method for detecting wine-related microorganisms was very sensitive, and it can detect 10
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