Evidence map›Paper›PMID 42243109›Full record

ArticleNature communications2026

ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity.

Richard R Spinks, Aleksa Lakic, Celine Kelso, Slobodan Jergic, Olga Yurieva, Zhi-Qiang Xu, Michael E O'Donnell, Nicholas E Dixon, Antoine M van Oijen, Jacob S Lewis and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Richard R Spinks *Molecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.
Aleksa Lakic *Molecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.ORCID 0009-0008-4131-1215
Celine KelsoMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.ORCID 0000-0003-0032-9650
Slobodan JergicMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.
Olga YurievaLaboratory of DNA Replication, Rockefeller University, New York, NY, USA.
Zhi-Qiang XuMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.ORCID 0000-0002-5884-0569
Michael E O'DonnellLaboratory of DNA Replication, Rockefeller University, New York, NY, USA.
Nicholas E DixonMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia.ORCID 0000-0002-5958-6945
Antoine M van OijenMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia. vanoijen@uow.edu.au.ORCID 0000-0002-1794-5161
Jacob S LewisMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia. jacobl@uow.edu.au.ORCID 0000-0002-9945-6133
Lisanne M SpenkelinkMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW, Australia. lisanne@uow.edu.au.ORCID 0000-0002-5511-8757

Funding

Biochemistry of Eukaryotic Replication Fork and DNA RepairR35GM148159 · NIGMS · ROCKEFELLER UNIVERSITY · PI MICHAEL E O'DONNELL · 2023 to 2026
$1.7M
Department of Education and Training | Australian Research Council (ARC) DE240100780Department of Education and Training | Australian Research Council (ARC) DP180100858, DP210100067Department of Education and Training | Australian Research Council (ARC) DP180100858, DP210100067, FL140100027Department of Health | National Health and Medical Research Council (NHMRC) 1197069Department of Health | National Health and Medical Research Council (NHMRC) 2007778Department of Health | National Health and Medical Research Council (NHMRC) 2025412NIGMS NIH HHS R35 GM148159
6 · The paper itself

Abstract

Adenosine triphosphate (ATP) hydrolysis is the main cellular source of energy used to drive biochemical reactions that are otherwise energetically unfavourable. The chemical energy stored in phosphoanhydride bonds is released upon hydrolysis of ATP to ADP and is used to drive mechanical work and conformational change. DNA replication is a canonical process in which the multi-enzyme replisome is thought to rely on ATP hydrolysis for its function. Here we show, through single-molecule visualisation of DNA replication by the Escherichia coli replisome, that the replicative DnaB helicase does not rely on hydrolysis of ATP in the context of the elongating replisome. Even in the presence of physiologically-relevant concentrations of ATP, dTTP is hydrolysed preferably. Finally, we show that the replicative helicases from S. cerevisiae, D. melanogaster, and Homo sapiens can also use dTTP to unwind DNA. Our observations suggest that replicative helicases across domains of life are 'flex-fuel' helicases.

Indexed as

Adenosine TriphosphateDnaB HelicasesDNA HelicasesDNA ReplicationEscherichia coliEscherichia coli ProteinsHumansHydrolysisSaccharomyces cerevisiaeThymine NucleotidesAdenosine TriphosphateDnaB HelicasesdnaB protein, E coliDNA HelicasesEscherichia coli Proteinsthymidine 5'-triphosphateThymine Nucleotides

Identifiers

PMID42243109
PMCPMC13276069

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.